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19319 results for Assays

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Assays

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.

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Cell viability assay kits

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

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Bicinchoninic acid (BCA) protein assay

Bicinchoninic acid (BCA) protein assay

Supplier: G-Biosciences

The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.

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Adipogenesis assay kit

Supplier: ENZO LIFE SCIENCES

Convenient method for the enhanced induction and detection of adipogenesis, in the classic 3T3L-1 model.

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OXI-TEK TBARS assay kit

OXI-TEK TBARS assay kit

Supplier: ENZO LIFE SCIENCES

The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress.

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VWR®, Bradford protein assay, proteomics grade

VWR®, Bradford protein assay, proteomics grade

Supplier: VWR Chemicals

The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.

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APO-BRDU™ kit, TUNEL Assay

Supplier: ENZO LIFE SCIENCES

Cellular assay, APO-BRDU™ kit

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p53 transcription factor assay kit

p53 transcription factor assay kit

Supplier: Cayman Chemical

96-Well assay for measurement of p53.

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Calcineurin phosphatase assay kit

Supplier: ENZO LIFE SCIENCES

This colorimetric kit is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. Activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.

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PPARα transcription factor assay kit

PPARα transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARα DNA binding activity.

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Protein carbonyl colorimetric assay kit

Supplier: Cayman Chemical

The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.

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Cellular senescence activity assay

Cellular senescence activity assay

Supplier: ENZO LIFE SCIENCES

Highly reproducible SA-β-gal activity kit

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Neutrophil/Monocyte Respiratory Burst Assay Kit

Neutrophil/Monocyte Respiratory Burst Assay Kit

Supplier: Cayman Chemical

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

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PPARδ transcription factor assay kit

PPARδ transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARδ DNA binding activity.

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Calcineurin cellular activity assay kit

Calcineurin cellular activity assay kit

Supplier: ENZO LIFE SCIENCES

A complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS human recombinant calcineurin is included as a positive control.

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TUNEL assay kits

TUNEL assay kits

Supplier: Biotium

TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

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Micro Bicinchoninic Acid (BCA) protein assay

Micro Bicinchoninic Acid (BCA) protein assay

Supplier: G-Biosciences

The Micro Bicinchoninic Acid Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions. This modification of the Bicinchoninic Acid (BCA) Protein Assay is suitable for dilute protein samples (0,5 to 20 µg/ml).

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Calcein-AM cell viability assay kits

Calcein-AM cell viability assay kits

Supplier: Biotium

Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.

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Cellular senescence live cell analysis assay

Supplier: ENZO LIFE SCIENCES

Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.

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DPPIV/CD26 assay kit for biological samples

Supplier: ENZO LIFE SCIENCES

The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered in vivo.

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NF-κB (p65) transcription factor assay kit

NF-κB (p65) transcription factor assay kit

Supplier: Cayman Chemical

NF-κB (p65) Transcription Factor Assay Kit is a 96-well assay for measurement of NF-κB (p65).

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LDH Cytotoxicity WST Assay

Supplier: ENZO LIFE SCIENCES

Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.

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Calcium assay kits, FLIPR® Calcium 4 assay kit

Calcium assay kits, FLIPR® Calcium 4 assay kit

Supplier: MOLECULAR DEVICES

The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.

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GFP-CERTIFIED® FLUOFORTE® Calcium assay kit

Supplier: ENZO LIFE SCIENCES

The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

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ER-ID™ green assay kit

Supplier: ENZO LIFE SCIENCES

The ER-ID® Green assay kit is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.

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Calcium assay kits, FLIPR® Calcium 5 assay kit

Calcium assay kits, FLIPR® Calcium 5 assay kit

Supplier: MOLECULAR DEVICES

The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.

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GOLGI ID® Green assay kit

Supplier: ENZO LIFE SCIENCES

The GOLGI ID® Green assay kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. It is more faithfully localized to the target organelle, with minimal staining of the endoplasmic reticulum. Micromolar concentrations of GOLGI ID® Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS.

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ApoSENSOR™ ADP/ATP ratio assay kit

Supplier: ENZO LIFE SCIENCES

The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.

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ProteoStat™ PDI assay kit

Supplier: ENZO LIFE SCIENCES

The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.

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Fluor-de-Lys® SIRT1 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

A Fluor-de-Lys® fluorescent assay system. The SIRT1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT1 included in the kit.

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ER-ID™ red assay kit (GFP-CERTIFIED®)

ER-ID™ red assay kit (GFP-CERTIFIED®)

Supplier: ENZO LIFE SCIENCES

ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.

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