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Bio-assayt

Bio-assayt

Bio-assayt (testit, määritykset) ovat laboratoriolääketieteessä, farmakologiassa, ympäristöbiologiassa, molekyylibiologiassa sekä proteomiikassa yleisesti käytettäviä testejä. Assayden avulla näytteiden funktionaalista aktiviteettia sekä pitoisuuksia voidaan määrittää kvalitatiivisesti tai kvantitatiivisesti. Tutkittavasta näytteestä mitattava emissio syntyy usein esim. vasta-aineleimausta käyttäen joko kemiluminesenssin, bioluminesenssin tai fluoresenssin kautta. Bio-assayt mahdollistavat perinteisiä kemikaalisia analyysejä monipuolisemman biologisten materiaalien käyttäytymisen sekä niiden kvantitoinnin tutkimisen. VWR:n valikoimasta löydät kaikki tärkeimmät bioassayt:
- Solutestit (Cellular assays)
- Entsyymivälitteiset immunosorbenttimääritykset (ELISA-assays)
- Immunologiset testit
- Proteiinitestit
- Kvantitatiivinen PCR (qPCR)

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Bicinchoninic acid disodium salt hydrate

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

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Glucose (HK) assay kit

Supplier: Merck

The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.

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VWR® Fluorescent DNA Quantitation Kit

VWR® Fluorescent DNA Quantitation Kit

Supplier: VWR Chemicals

Fluorescent DNA Quantitation Kit uses fluorescent bisBenzimide H33258 dye to bind to AT sequences of the minor groove of double-stranded DNA. In the assay, the sample is excited at 360 nm and when the dye binds to dsDNA an emission spectrum at 460 nm is produced.

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VWR®, Bradford protein assay, proteomics grade

VWR®, Bradford protein assay, proteomics grade

Supplier: VWR Chemicals

The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.

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Human CD62E ELISA Set (without plates)

Human CD62E ELISA Set (without plates)

Supplier: Abcam

Human CD62E ELISA Set (without plates) is a Sandwich (quantitative) ELISA kit for the measurement of Human CD62E ELISA Set (without plates) in Human in Plasma, Cell culture supernatant, Serum samples.

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Nitrotyrosine IP Kit

Nitrotyrosine IP Kit

Supplier: Cayman Chemical

For the capture and pulldown of nitrated proteins from cell and tissue lysates.

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Coxiella burnetii Phase 1 IgG ELISA Kit (Q-Fever)

Coxiella burnetii Phase 1 IgG ELISA Kit (Q-Fever)

Supplier: Abcam

Coxiella burnetii Phase 1 IgG ELISA kit (Q-Fever) is a Indirect ELISA kit for the measurement of Coxiella burnetii Phase 1 IgG (Q-Fever) in Human in citrate plasma, heparin plasma, serum samples.

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Human LKB1 ELISA Kit

Human LKB1 ELISA Kit

Supplier: Abcam

Human LKB1 ELISA kit is a single-wash 90-min Simplestep used to quantify Human LKB1 with a sensitivity of 1 ng/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. Colorimetric Sandwich ELISA - 450 nm readout: works on any standard plate reader.

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Human IL-8 ELISA Kit

Human IL-8 ELISA Kit

Supplier: Abcam

Human IL-8 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-8 (IL-8) with a sensitivity of 1,8 pg/ml. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric sandwich ELISA - 450 nm readout; compatible with any standard plate reader - Available in different formats to meet various throughput needs, including 384-well plates for higher throughput - Broad sample compatibility – suitable for serum, plasma, cell culture supernatants, and cerebrospinal fluid - Cited in over 30 publications.

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Human Colipase ELISA Kit

Human Colipase ELISA Kit

Supplier: Abcam

Human Colipase ELISA kit is a single-wash 90-min Simplestep used to quantify Human Colipase with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. Colorimetric Sandwich ELISA - 450 nm readout: works on any standard plate reader.

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Human Transferrin ELISA Kit

Human Transferrin ELISA Kit

Supplier: Abcam

Human Transferrin ELISA kit is a single-wash 90-min Simplestep used to quantify Human Transferrin with a sensitivity of 4,396 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. Colorimetric Sandwich ELISA - 450 nm readout: works on any standard plate reader. Different formats for different needs: 384-well plate for higher throughput.

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Human ACE2 ELISA Kit

Human ACE2 ELISA Kit

Supplier: Abcam

Human ACE2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human ACE2 with a sensitivity of 1052 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader. - Cited in over 20 citations.

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Pig IgG ELISA Kit

Pig IgG ELISA Kit

Supplier: Abcam

Pig IgG ELISA Kit is a ELISA kit for the measurement of Pig IgG in Pig in Plasma, Serum samples.

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Mouse IFNGR1 ELISA Kit

Mouse IFNGR1 ELISA Kit

Supplier: Abcam

Mouse IFNGR1 ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Mouse IFNGR1 in Mouse in Plasma, Cell culture media, Serum samples.

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Rat IL-6 ELISA Kit

Rat IL-6 ELISA Kit

Supplier: Abcam

Rat IL-6 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Rat IL-6 in Rat in plasma, cell culture supernatant samples.

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Human TNF alpha ELISPOT Kit

Human TNF alpha ELISPOT Kit

Supplier: Abcam

Human TNF alpha ELISPOT Kit is a ELISPOT Kit for the sensitive detection of Human TNF alpha production in a single cell suspension.

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