Next Generation Sequencing Reagents

Supplier: Oxford Nanopore Technologies

Amplification and full-length sequencing of 16S rRNA for bacterial profiling and ITS for fungal profiling, with barcoding for up to 24 samples.

Supplier: Biotium

The AccuBlue® NextGen dsDNA Quantitation Kit offers unprecedented level of sensitivity in dsDNA detection. It is a sensitive and accurate DNA quantitation method for ultra-low amounts of DNA with a detection limit as low as one pg DNA in a 96-well plate reader assay.

Supplier: Abcam

High Sensitivity DNA Library Preparation KIt (ab185905) is a complete set of optimized reagents to prepare a DNA library from very small amount of samples for use in next-generation sequencing applications.

Supplier: Abcam

ab185906 is a complete set of optimized reagents to prepare a DNA library - after successful bisulfite conversion - for various Illumina® platform-based bisulfite sequencing (bisulfite-seq) assays, such as whole genome bisulfite sequencing (WGBS), oxidative bisulfite sequencing (oxBs-seq), reduced representative bisulfite sequencing (RRBS), and other bisulfite-based next generation sequencing applications.

Supplier: OMEGA BIO-TEK

Mag-Bind® TotalPure NGS offers an easy-to-use, reliable solution for the purification of both DNA and RNA for next-generation sequencing workflows with high recovery rates. Mag-Bind® TotalPure NGS is capable of selectively binding fragments depending on the reagent to sample ratio used, giving the user flexibility to perform left, right or double-sided size selection.

Supplier: Quantabio

An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.

Supplier: Quantabio

sparQ HiFi PCR Master Mix was specially designed for high efficiency library amplification from low DNA input. sparQ HiFi PCR Master Mix minimises the number of amplification cycles required to achieve the threshold required for sequencing. The result is a superior performing high fidelity master mix that increases library yield which reduces PCR-derived artifacts for a variety of sequencing applications.

Supplier: Quantabio

The sparQ DNA Frag and Library Prep kit optimises the integration of enzymatic fragmentation into a two-step protocol for the streamlined construction of libraries for sequencing on Illumina® NGS platforms. A single-tube enzyme mix facilitates the combination of fragmentation and DNA polishing minimising DNA over fragmentation while greatly simplifying library.

Supplier: Quantabio

sparQ PureMag beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

Supplier: Quantabio

sparQ Universal Library Quant Kit provides rapid and accurate quantification of libraries prepared for sequencing on Illumina® NGS platforms.

Supplier: Oxford Nanopore Technologies

DNA or RNA for sequencing control experiments.

Supplier: Oxford Nanopore Technologies

PCR Barcoding Expansion packs enable multiple nanopore sequencing libraries to be pooled and run on a single MinION Flow Cell.

Supplier: Oxford Nanopore Technologies

Wash and reuse MinION Flow Cells to maximise output and reduce cost per sample.

Supplier: Oxford Nanopore Technologies

PCR Primers (PRM) and PCR Adapters (PCA).

Supplier: Oxford Nanopore Technologies

Short Fragment Buffer (SFB).

Supplier: Oxford Nanopore Technologies

An expansion kit containing all the required reagents and primers to generate reverse-transcribed and PCR-amplified material from SARS-CoV-2 RNA samples.