Hait: Cell Culture Supplements and Additives

Supplier: Thermo Fisher Scientific

Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Supplier: Charles River Laboratories Cell Solutions

HypoThermosol® biopreservation media is a novel, engineered, optimised hypothermic storage and shipping media product. Serum-free, protein-free HypoThermosol® is designed to provide maximum storage and shipping stability for biologics at 2 to 8 °C.

Supplier: PanReac AppliChem

Yeast extract BioChemica

Supplier: Corning

Interleukin-3 (IL-3) is a glycoprotein that regulates differentiation and enhances proliferation of a variety of hematopoietic precursor cells, mast cells, basophils, and NK cells.

Supplier: Corning

Corning® cellgro® COMPLETE formulation is based on a proprietary combination of 1:1 DMEM/F12, RPMI and McCoy's 5A medium. This medium contains a mixture of selected trace elements and high molecular weight carbohydrates, extra vitamins, animal origin-free protein, and a small amount of high quality bovine serum albumin (1,0 g/L). The Corning cellgro COMPLETE supports superior growth and viability over long-term passages in hybridomas, along with suspension and adherent cell cultures.

Supplier: Thermo Fisher Scientific

Phosphorylation and dephosphorylation is a molecular on/off switch that regulates a number of key biological pathways within the cell, including signal transduction, cell division, and apoptosis. The Halt Phosphatase Inhibitor Cocktail preserves phosphorylation of proteins in cell lysates and tissue extracts.

Supplier: STEMBIOSYS

CELLvo™ XF Matrix is a ready to use cell culture substrate which provides a xeno-free native microenvironment that replicates the 3-dimensional 'home' in which stem cells naturally reside and proliferate.

Supplier: STEMBIOSYS

CELLvo™ Matrix Plus is a substrate that aims to recapitulate the biochemical, structural and mechanical cues of the native niche by leaving the extracellular matrix intact. Cell-derived matrices create a biologically relevant culture environment for a variety of cell types including pluripotent cells by providing all the cues cells expect to receive in vivo in culture dishes.

Supplier: STEMBIOSYS

CELLvo™ Matrix is a ready to use cell culture substrate which provides a native microenvironment that replicates the 3-dimensional 'home' in which stem cells naturally reside and proliferate.

Supplier: Corning

Corning® Nu-Serum™ IV is a growth medium supplement that provides a low-protein alternative to newborn calf, fetal bovine, and other sera routinely used for cell culture.

Supplier: Corning

ITS contains insulin, human transferrin, and selenous acid, the three most universally essential components of defined culture media.

Supplier: Corning

MITO+ Serum Extender is a concentrated, fully defined formulation of hormones, growth factors (EGF and FGF), and other metabolites (insulin and steriod hormones).

Supplier: Corning

BPE is a broadly used supplement to culture a variety of epithelial and endothelial cells.

Supplier: Corning

Endothelial Cell Growth Supplement (ECGS) is a broadly used supplement to culture a variety of cells, particularly endothelial cells.

Supplier: Corning

T-STIM with ConA (IL-2 culture supplement), rat, is used to promote proliferation and activation of T-cells and support high-titer HIV production by leukocytes.

Supplier: Thermo Fisher Scientific

These inhibitor tablets contain broad-spectrum protease inhibitors that are highly effective at preventing proteolytic degradation during cell lysis and protein extraction experiments.

Supplier: Corning

Pre-screned for compatibility with mTeSR™1 medium by StemCell Technologies providing the reproducibility and consistency essential for hESC and iPSC feeder-free stem cell culture.

Supplier: Corning

Cells grown in traditional media supplemented with L-glutamine are susceptible to toxicity.

Supplier: Corning

The addition of MEM nonessential amino acids to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells.

Supplier: Thermo Fisher Scientific

The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts.

Supplier: Corning

Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.

Supplier: Corning

An in vitro system for assessing the invasive potential of both malignant and normal cells.

Supplier: Corning

The addition of MEM vitamins to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells.

Supplier: Corning

Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.

Supplier: Corning

In our Starter Kit we have combined 30 ml of Corning 3D Clear with the four buffer solutions required to process 3D tissue cultures for immunolabelling. All you need to do is add your immunolabels and nuclear stains. This kit comes with enough material to process three 96-well plates of 3D cell culture models.

Supplier: Corning

Corning® Cell Recovery Solution allows for the recovery of cells cultured on Corning® Matrigel® Basement Membrane Matrix for subsequent biochemical analysis. Corning® Cell Recovery Solution depolymerises Corning® Matrigel® Matrix gels without enzymatic digests and lengthy incubation periods at high temperatures. Cells are released without damage thereby avoiding biochemical changes during incubation and digestion of extracellular portions of cell-surface receptors and adhesion molecules.

Supplier: HyClone products (Cytiva)

The HyClone Cell Boost™ process supplement kit contains 6×100 g samples of supplements designed to increase cell productivity in a variety of cell lines. Each supplement is designed to provide nutrients to fed-batch culture applications.

Supplier: Merck Millipore (Calbiochem‎)

Calbiochem® protease inhibitor cocktail set III, animal-free, is a cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine and serine proteases and aminopeptidases.

Supplier: Corning

Animal free formulation

Supplier: Thermo Fisher Scientific

Phosphatase Inhibitor Cocktail I is used to inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases.