You searched for: Cell Culture Supplements and Additives

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement used primarily for human cells and the production of cell secreted proteins. TCH™ is also effective in culturing other mammalian cells without the use of animal proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, recombinant insulin), which are heat treated.

Supplier: G-Biosciences

Recom ProteaseArrest™ is a broad range, bacterial, 100X concentrated, ready to use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Recom ProteaseArrest™ inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

Supplier: G-Biosciences

ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.

Supplier: VWR Chemicals

Egg yolk emulsion, sterile, VWR®

Supplier: BIOWEST

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: PROVITRO

Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the tube. After usage store them in the dark at 2 °C to 8 °C for a maximum of 2 weeks. Take care: After thawing shelf time of Dispase II solution is limited to 14 days.

Supplier: G-Biosciences

The ProteaseArrest™ family of protease inhibitor cocktails are supplied as convenient, ready-to-use, liquid format cocktails that offer immediate >95% inhibition of harmful proteases. The 100X concentrated solution outperforms other similar protease inhibitor cocktails (including tablet formats) by offering instant inhibition, allowing delivery of optimised concentrations, demonstrating higher inhibition levels, and providing a greater ease of use. Also available as single use OneQuant™ ProteaseArrest™ aliquots.

Supplier: Thermo Fisher Scientific

Halt protease and phosphatase inhibitor cocktail provides the convenience of a single solution with full sample protection. This broad-spectrum inhibitor cocktail is specifically optimised to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.

Supplier: G-Biosciences

TCM-ProteaseArrest™ is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic (acid) proteases and aminopeptidases.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: THERMO FISHER DIAGNOSTICS

Egg yolk emulsion

Supplier: HyClone products (Cytiva)

Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, salts, trace elements, and glucose. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: BIOWEST

D-glucose monohydrate (Dextrose) is a common natural sugar involved in processes such as energy production, glycosylation, and formation of glycans that provide structure to cells. It is involved in a detrimental process in cells called glycation. It is used as a supplement for cell culture and in numerous cellular processes.

Supplier: BIOWEST

Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Yeast/ Fungal ProteaseArrest™ inhibits yeast and fungal serine, cysteine and metalloproteases.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Mammalian ProteaseArrest™ inhibits mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases.

Supplier: PROTIDE PHARMACEUTICALS

When combined with DMSO, 2-8CELLsius™ can be utilised as a cryopreservation solution. 2-8CELLsius™ + DMSO is a cytoprotective, protein-free solution, with bio-stabilisers that support high post thaw cell viability. Optimal recovery is achieved through a novel approach to cell physiology at ultra-low temperatures.

Supplier: PROVITRO

The supplement kit for microvascular endothelial cell growth medium, advanced is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: PROTIDE PHARMACEUTICALS

Ready to use, balanced, cytoprotective, protein-free solution with bio-stabilisers for short-term storage, regenerative medicine, stem cell processing and transportation of biological material. Short-term storage can be achieved at 2 to 8 °C or at ambient temperature for 24 to 48 hours. Does not contain any growth factors, hormones, or antibiotics. 2-8CELLsius™ can also be utilised as a cell washing solution.

Supplier: Thermo Fisher Scientific

Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Supplier: THERMO FISHER DIAGNOSTICS

Egg yolk tellurite emulsion

Supplier: HyClone products (Cytiva)

Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

SG-200 solution is a stable dipeptide of L-alanyl L-glutamine.

Supplier: Apollo Scientific

Inhibits aspartic, serine, cysteine and metalloproteases.

Supplier: BIOWEST

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge the cells from their substrate depends on the sensitivity of the cells.

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Bacterial ProteaseArrest™ inhibits bacterial serine, cysteine and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

Supplier: PROVITRO

The supplement kit for microvascular endothelial cell growth medium is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

Supplier: PROVITRO

The supplement kit for endothelial cell proliferation medium is suitable for culturing Provitro human endothelial cells after adding the supplement kit components to 500 ml basal medium The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only. The supplement kit for endothelial cell priliferation medium is thoroughly tested after each production. All components are tested in a stringent biological assay. Each batch is checked for human endothelial cells proliferating characteristics. The cells cultured in endothelial cell growth medium are checked regarding their morphology, the adherence rate, the colony forming efficiency and the population doubling time.