You searched for: Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
NP-40 lysis buffer, low salt
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, low salt, Thermo Fisher Scientific
Expand 2 Items
HEPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
Expand 1 Items
Succinate, 0,2 M buffer solution, pH 5,0
Supplier: Thermo Fisher Scientific
Succinate, 0.2M buffer soln., pH 5.0, Thermo Fisher Scientific
Expand 2 Items
Protein extraction reagent, BugBuster® 10X, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.
Expand 2 Items
Triton® X-100 lysis buffer with glycerol (2X), pH 7,4
Supplier: Thermo Fisher Scientific
Triton® X-100 lysis buffer with glycerol (2X), pH 7.4, Thermo Fisher Scientific
Expand 1 Items
Protein extraction reagents, BugBuster® and BugBuster® plus Benzonase®, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.
Expand 3 Items
Protein extraction reagent, BugBuster® HT, Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.
Expand 3 Items
2-D Protein Extraction Buffer
Supplier: Cytiva
Efficient cell lysis and protein extraction are key steps for achieving high-quality results in downstream applications. The 2-D protein extraction buffer trial kit is designed for those who experiment for establishing a suitable extraction and solubilization buffer for their protein samples.
Expand 1 Items
CSPL buffer
Supplier: OMEGA BIO-TEK
Component of the Mag-Bind® plant DNA 96 plus kit.
Expand 1 Items
Glucose hemolysing solution
Supplier: DIAGNOSTIC SYSTEMS DIASYS
For use with glucose Gluc-DH FS and glucose Gluc-DH FSC.
Expand 1 Items
EasySep™ Red Blood Cell Lysis Buffer
Supplier: STEMCELL TECHNOLOGIES
EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.
Expand 2 Items
Ammonium Chloride Solution
Supplier: STEMCELL TECHNOLOGIES
Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.