You searched for: Extraction Buffers
Extraction buffers are provided ready to use and are effective in the isolation of total RNA and other proteins, improving protein yields and sample complexity from cells and tissues. Optimized extraction reagents enhance protein extraction in native or denaturing buffers compatible with downstream analytical techniques. An appropriate buffer solution added to a protein mixture during the extraction process can help improve the stability of protein molecules as these molecules are subjected to various forces designed to isolate them for study.
Y-PER™ Yeast Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.
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Protein extraction kit, bacterial PE LB™
Supplier: G-Biosciences
Bacterial PE LB™ kit has been developed for the extraction of biologically active soluble proteins, including recombinant proteins and inclusion bodies, from bacterial cells. Bacterial PE LB™ is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA and RNA) released during cell lysis. The Bacterial PE LB™ lysis system eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.
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Protein extraction kit, tissue PE LB™
Supplier: G-Biosciences
Tissue PE LB™ has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue PE LB™ is based on an organic buffer and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue PE LB™. Tissue PE LB™ has been tested for use with a wide variety of fresh and frozen animal tissues.
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ReadiUse™ Bacterial Cell Lysis Buffer *5X* (10 ml)
Supplier: AAT BIOQUEST
Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules.
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Protein extraction kit, mammalian cell PE LB™
Supplier: G-Biosciences
Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.
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Subcellular Protein Fractionation Kit for Cultured Cells
Supplier: Thermo Fisher Scientific
This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.
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Subcellular protein fractionation kit for tissues
Supplier: Thermo Fisher Scientific
This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.
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Protein extraction kit, Insect PE LB™
Supplier: G-Biosciences
Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.
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I-PER™ Insect Cell Protein Extraction Reagents
Supplier: Thermo Fisher Scientific
The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.
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Nuclear and cytoplasmic extraction reagents, NE-PER
Supplier: Thermo Fisher Scientific
The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.
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RBC lysis buffer for human
Supplier: Thermo Fisher Scientific
RBC lysis buffer for human, Thermo Fisher Scientific
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T-PER™ Tissue Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.
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RIPA Lysis and Extraction Buffer
Supplier: G-Biosciences
A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.
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NP-40 lysis buffer
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, Thermo Fisher Scientific
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Cell lysis buffers
Supplier: Rockland Immunochemicals
Cell lysis buffer is used to lyse cells under non-denaturing conditions.
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Triton® X-100 lysis buffer, pH 7,4
Supplier: Thermo Fisher Scientific
Triton® X-100 lysis buffer, pH 7.4, Thermo Fisher Scientific
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NP-40 lysis buffer with glycerol (2X)
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer with glycerol (2X), Thermo Fisher Scientific
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HEPES protein extraction buffer (5X)
Supplier: Thermo Fisher Scientific
Thermo Fisher Scientific
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CHAPS lysis buffer
Supplier: Thermo Fisher Scientific
For cell lysis and protein extraction.
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Denaturing lysis buffer
Supplier: Thermo Fisher Scientific
Denaturing lysis buffer, Thermo Fisher Scientific
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Protein extraction reagent, BugBuster® (primary amine-free), Millipore®
Supplier: Merck Millipore (Novagen)
BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.
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RBC lysis buffer for mouse
Supplier: Thermo Fisher Scientific
RBC lysis buffer for mouse, Thermo Fisher Scientific
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HEPES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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MNE buffer, pH 6,5
Supplier: Thermo Fisher Scientific
MNE buffer, pH 6.5, Thermo Fisher Scientific
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MES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
MES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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NP-40 lysis buffer (2X)
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer (2X), Thermo Fisher Scientific
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Lysis buffers, red blood cells
Supplier: G-Biosciences
These DNase- and RNase-free buffers and reagents are designed for high quality research.
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PIPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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NP-40 lysis buffer, low salt
Supplier: Thermo Fisher Scientific
NP-40 lysis buffer, low salt, Thermo Fisher Scientific