For detection and measurement of human interleukin 2.

The Human Interleukin 2 (IL-2) ELISA Kit is designed for the quantitative detection and measurement of human IL-2 in biological fluids such as serum, plasma, and cell culture supernatants. IL-2 is primarily secreted by activated CD⁴⁺ and CD⁸⁺ T cells, but is also produced by B cells and dendritic cells in smaller amounts.

IL-2 is a major regulator of T cell development, contributing to T cell growth and expansion, as well as the development of regulatory T cells. The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine.

The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a coloured product with an intensity directly proportional to the concentration of cytokine in the sample.

The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analysed in parallel.