Mouse nerve growth factor 25S (from submaxillary glands)
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About this item
Isolated from mouse submaxillary glands by a modification of the procedure described by T. Darling, et al.; Cell Culture Meth. Mol. Cell Biol. 4, 79 (1984).
Ordering information: Quantity: Concentration is estimated spectroscopically from its extinction coefficient and amino acid analysis.
Specifications
- CAS No.:93828-24-6
- Source:Submaxillary glands
- Species:Mouse
- Storage conditions:−20 °C
- Biological activity:Neurotrophic activity was bioassayed using rat pheochromocytoma cell PC12 cultures over a period of 7 - 14 days. Activity is in the range of 0,5 - 50 ng/ml. Recommended concentration to be used in vitro for maintenance of sympathetic and sensory nerve cultures is 50 ng/ml medium.
- Reconstitution instructions:Dilute in sterile water or DMEM to a concentration of at least 10 µg/ ml and store at –80 °C before use.
- Protein synonyms:NGF 2.5S
- Protein/peptide name:Nerve Growth Factor 25S
- Purity:>90% (SDS-PAGE)
- Molecular weight:~26kDa; homodimer
- Formulation:Lyophilised