Specifications
- Antibody type:Primary
- Antigen name:Cleavage Stimulation Factor, 3' pre-RNA, Subunit 3, 77kDa
- Antigen symbol:CSTF3
- Clonality:Polyclonal
- Conjugation:Unconjugated
- Host:Rabbit
- ImmunoChemistry:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western blot:Yes
- Form:liquid
- Gene ID:1479
- Antigen synonyms:MGC75122|CSTF 77 kDa subunit|MGC117398|cleavage stimulation factor 3' pre-RNA subunit 3 77kDa|cleavage stimulation factor subunit 3 isoform 1|cleavage stimulation factor subunit 3|MGC43001|CSTF-77
- Storage buffer:Aqueous buffered solution containing 100ug/ml BSA, 50% glycerol and 0,09% sodium azide. Store at -20°C for 12 months.
- Concentration:1 ug/ul
- Shipping temperature:4 °C
- Purification:Purified by Protein A.
- Size:100 μg
- Pk:100 µl
Specifications
About this item
Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits. Heterotrimeric CstF recognizes GU- and U-rich sequences located downstream of the polyadenylation site on RNA. CstF-77 (cleavage stimulation factor, 77 kDa subunit), also known as CstF3, is one of the three subunits comprising CstF. It can exist as a homodimer and functions as the bridge, directly interacting with the other two CstF subunits, namely CstF-64 and CstF-50. CstF-77 is highly conserved among eukaryotes. It contains an Alpha-helical structure with 11 HAT (Half-a-TPR-containing) repeats and is essential for CstF assembly. In addition, CstF-77 is capable of interacting with CPSF1 and FCP1, other factors involved in polyadenylation.
WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Type: Primary
Antigen: CSTF3
Clonality: Polyclonal
Clone:
Conjugation: Unconjugated
Epitope:
Host: Rabbit
Isotype:
Reactivity: