Human ICOS Ligand/ICOSL ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human ICOS Ligand/ICOSL in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutess, with a single wash step
• Detection Range: 400 to 6400 ng/L
• Sensitivity: 25,34 ng/L
• Sample Type: Serum, plasma or other biological fluids
• Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%

Human ICOS Ligand/ICOSL ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human ICOS Ligand/ICOSL in serum, plasma or other biological fluids. An antibody specific for ICOS Ligand/ICOSL has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the ICOS Ligand/ICOSL present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-ICOS Ligand/ICOSL antibody, which also binds the ICOS Ligand/ICOSL present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-ICOS Ligand/ICOSL antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-ICOS Ligand/ICOSL antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. colour develops in proportion to the amount of ICOS Ligand/ICOSL captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of ICOS Ligand/ICOSL in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.