Human Lin28B ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Lin28B in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutess, with a single wash step
• Detection Range: 1,25 to 20 ng/ml
• Sensitivity: 0,044 ng/ml
• Sample Type: Serum, plasma or other biological fluids
• Assay Precision: Intra-assay: CV <8%, Inter-assay: CV <10%

Human Lin28B ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Lin28B in serum, plasma or other biological fluids. An antibody specific for Lin28B has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Lin28B present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-Lin28B antibody, which also binds the Lin28B present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Lin28B antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Lin28B antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. Colour develops in proportion to the amount of Lin28B captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Lin28B in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.