- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:RNF111
- Description:Human RNF111 ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Human RNF111 ELISA Kit exhibits high specificity and excellent specificity for the detection of human RNF111. No significant cross-reactivity or interference between RNF111 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,156 - 10 ng/ml
- Storage temperature:4 °C
- Sensitivity:0,094 ng/ml
- Regulatory status:RUO
Human RNF111 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human RNF111 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Human RNF111 ELISA kit (A303048) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human RNF111 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for RNF111 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the RNF111 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-RNF111 Antibody, which binds the captured RNF111 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of RNF111 captured in each well. The concentration of RNF111 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.