Avantor offers a broad line of reagent droppers, diagnostic kits, and antigens and antisera, for aid in identifying a variety of microscopic organisms. The visualization of bacteria by means of staining procedures is an important tool for any microbiologist to have at their disposal, and these carefully standardized and reproducible stain reagents help to ensure accurate and easily interpretable results. Stains are also available to provide early detection of positive blood cultures by fluorescence procedures.
Araldite® M, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Araldite® is an epoxy resin commonly used for embedding. It is basically free from polymerization damage and the plastic does not sublime in the microscope, hence providing continual support and preservation of fine detail.[1] It is composed of araldite M or CY 212, dodecenyl succinic anhydride, dibutyl phthalate and 2,4,6-tridimethylamine methyl phenol.
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Zinc Formalin Fixative
Supplier: SIGMA ALDRICH MICROSCOPY
Zinc formalin fixative is a non-precipitating fixative. This fixative is compatible with histochemical, immunochemical and special stains. It may be used with automated tissue processors and also in manual methods. The zinc chloride component of the formulation increases the rigidity of cellular components to withstand subsequent processing, sectioning and staining procedures. Tissue specimens initially fixed using 10% neutral buffered formalin solution may be post-fixed using the zinc formalin fixative.
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Osteo-Bed Bone embedding kit, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Suitable for use with large and small mineralized (undecalcified) bone sections. Yields clear, hard blocks for cutting sections. Not water-soluble.
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Embedding and mounting media
Supplier: Merck
The defined hardness and uniformity of the material to be cut are vital requirements in creating consistent quality in cutting. These requirements can be met using a range of suitable substances, such as paraffins, resins, gelatine and celloidin.
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Decalcifier solutions, Osteomoll® and Osteosoft®, Sigma-Aldrich®
Supplier: Merck
Hard tissue like bones or teeth, and all specimens of calcified tissue like bone marrow punches require special pre-treatment before routine processing by a histological laboratory.
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VWR®, D.F.B (Decalcifying solution - H.K. Kristenson) Histological grade
Supplier: VWR Chemicals
Decalcifier according to Kristenson.
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J.T.Baker®, Mounting Medium, High, Histo Grade
Supplier: Avantor
Mounting medium for coverslipping of slides.
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VWR® HistoChoice®, Clearing agent
Supplier: VWR Chemicals
HistoChoice® clearing agent is a much less hazardous replacement for xylene/toluene.
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J.T.Baker® Ultrakitt, Mounting Medium, Histo Grade
Supplier: Avantor
Mounting medium for coverslipping of slides.
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Embedding medium, PolyFreeze, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.
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VWR® Q Path® O.C.T. Compound Mounting Medium for Cryotomy
Supplier: VWR Chemicals
High viscosity mounting medium for cryotomy.
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VWR® Q Path® Freeze Gel (Glue) Mounting Medium for Cryotomy
Supplier: VWR Chemicals
High viscosity mounting media for cryotomy. Less viscous than O.C.T.
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VWR® Gurr™ Histological Fixatives, AFA
Supplier: VWR Chemicals
Acidified alcoholic formalin for the fixation of glycogen.
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VWR® Q Path® DC3, Decalcifier Rapid-Acting
Supplier: VWR Chemicals
Contains a mixture of hydrochloric acid and alcohols with some EDTA.
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J.T.Baker®, Formalin Neutraliser
Supplier: Avantor
Formalin (dissolved formaldehyde) is used in histopathology laboratories.
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VWR® Fixing Solution
Supplier: VWR Chemicals
Fixing solution with 0,02% formaldehyde and 0,01% citric acid in aqueous solution.
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VWR® Q Path® Stick on (Cell adhesive) for Microscopy
Supplier: VWR Chemicals
Glue for improving adherance of histologicals sections to slides. Alternative to albumin-glycerol.
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VWR® Fixing Solution
Supplier: VWR Chemicals
Fixing solution with 0,02% formaldehyde in methanol 50% (v/v)).
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VWR®, Immersion Oil
Supplier: VWR Collection
Immersion oil is used to increase microscope resolution.
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Epoxy Embedding Medium Kit
Supplier: SIGMA ALDRICH MICROSCOPY
The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).
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VWR® Q Path® Easyfix, Cytology Fixative
Supplier: VWR Chemicals
Cytology fixative specially designed for the conservation and transport of cytology specimens.
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VWR®, OCT Embedding matrix for frozen sections
Supplier: VWR Chemicals
When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.
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VWR® Q Path® Cytofix, Cytology Fixture
Supplier: VWR Chemicals
Fixative, combining the coagulant qualities of alcohol, to enable a better fixation of nuclear and cytoplasmic elements. The erythrocytes are lysed and, therefore, do not obscure observation of the elements of interest. This fixative can be used according to the Saccomanno method.
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VWR® Q Path®, Slow Acting Decalcifiers
Supplier: VWR Chemicals
DC1 slow acting decalcifiers contain a mixture of formic acid and formalin.
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VWR® Q Path® Safemount, Mounting Agents for Microscopy
Supplier: VWR Chemicals
Safemount Q Path® is a mounting medium, supplied in an PET bottle, which is compatible with Safesolv. It glues the coverglass to the slide and fixes the probe in position after staining.
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VWR® Cryolab Q Path, Freezing Aerosol
Supplier: VWR Chemicals
Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.
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VWR® Q Path®, Medium-acting decalcifiers
Supplier: VWR Chemicals
This medium-acting decalcifier contains a mixture of hydrochloric acids.
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VWR® Q Path® Coverquick, Mounting Agents for Microscopy
Supplier: VWR Chemicals
A range of mounting media for manual and automatic mounting of slides. The Q Path® Coverquick range consists of four different mounting media with different viscosities to meet all microscopy needs. The number after the name indicates increasing viscosity: Q Path® Coverquick 1000 has the lower viscosity (around 260 mPaS) and Q Path® Coverquick 4000 has the higher viscosity (around 920 mPaS).
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Mounting medium, Entellan® new
Supplier: Merck
Entellan® new is a new mounting medium for cover slippers and is specially suited for commercial automated mounting instruments that operate with cover slippers. Its viscosity ranges between 250 and 600 mPas, 1,00869 has a small viscosity range. This narrow viscosity range improves and accelerates the application, as neither the dropping rate nor the volume need to be reset after the first run with a new bottle of Entellan® new.