"protein purification system"

Supplier: TOSOH Bioscience

TOYOPEARL® Super A resin provides a cutting-edge experience in protein A media, blending top-tier critical performance attributes with exceptional operational flexibility. An efficient and durable protein A resin.

Supplier: Cytiva

Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.

Supplier: G-Biosciences

The concentrator is a high molecular weight polymer which will not migrate across the dialysis membrane. The concentrator is placed against the dialysis membrane of the Tube-O-DIALYZER™, where it rapidly absorbs water from the sample and reduces the sample volume. The concentrator can be used with dialysis bags and other dialysis devices.

Supplier: G-Biosciences

The HOOK™ Cell Surface Protein Isolation Kit uses HOOK™ biotin labelling and purification technology in conjunction with Mammalian Cell PE LB™ Lysis Buffer to conveniently label cell surface proteins and isolate them for further analysis, including Western blotting. The Kit is supplied with all the necessary reagents and buffers for convenience and improved reproducibility. The kit is compatible with a wide variety of mammalian cells and can be used to compare treated and untreated cells and differences between different cell lines.

Supplier: G-Biosciences

G-Biosciences offers a wide selection of genomic isolation kits that purify high quality genomic DNA from a variety of sources and for a wide array of applications. G-Biosciences XIT™ DNA kits produce protein-free, high quality DNA through the principle of cell lysis, protein digestion and precipitation and genomic DNA purification. No chloroform or phenol extraction is required. High quality DNA can be isolated from sample types including: animal tissues, cells, whole blood, bacteria, buccal cells, plant tissues, mouse tail, yeast and FFPE tissue. G-Biosciences XIT™ DNA kit procedures remove contaminants and enzyme inhibitors allowing the purified DNA to be ready for immediate use for all downstream analyses. The purified DNA from G-Biosciences XIT™ DNA kits have a A₂₆₀/A₂₈₀ ratio between 1,7 and 1,9 (with the exception of the buccal cells kit with a ratio between 1,8 and 2,0) and are up to 200 kb in size.

Supplier: Cytiva

The illustra™ triplePrep Kit is designed for the rapid, simultaneous extraction and isolation of high yield genomic DNA (gDNA), total RNA, and total denatured proteins from undivided animal tissues and mammalian cells. The streamlined workflow reduces the overall number of steps, enabling the preparation of all three analytes in less than one hour.

Supplier: Cytiva

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

Supplier: Cytiva

NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.

Supplier: Abcam

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. ab102784 quickly removes these contaminants.

Supplier: Thermo Fisher Scientific

SMART Digest™ Chymotrypsin Kits are designed to provide a complimentary digestion option to the SMART Digest™ Trypsin Kits which incorporate trypsin functionality. The Chymotrypsin enzyme functionality allows digestion of proteins at different points of the amino acid chain compared to trypsin. This allows for different/additional structural information to be obtained when trying to characterise a protein. This option can also be used if a protein does not contain the specific point where trypsin cuts the protein chain.

Supplier: Thermo Fisher Scientific

The Direct Magnetic IP/Co-IP Kit uses NHS-chemistry to covalently immobilise IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ Proteinase K Kits are designed to provide a simple to use, fast, highly reproducible and robust process for digestion of proteins in a high throughput format, meeting the needs of biopharmaceutical, bioanalytical and QA/QC environments. This version of the kit utilises Proteinase K, which cleaves the peptide bond adjacent to the carboxyl gropu of aliphatic and aromatic amino acids with blocked alpha amino groups.

Supplier: Cytiva

RESOURCE Q columns are prepacked with SOURCE 15Q, a strong anion exchanger for high resolution polishing purification of proteins at high flow rates.

Supplier: ELGA LabWater

Ultra filtration cartridge, ENDOGARD, 5000 MWCO, removes particles as small as protein macromolecules

Supplier: Thermo Fisher Scientific

KingFisher Ready DNA Ultra 2.0 Prefilled plates are automation-ready plates prefilled with MagMAX DNA Multi-Sample Ultra 2.0 reagents suitably configured for KingFisher Flex purification systems. KingFisher Ready DNA Ultra 2.0 prefilled plates enable isolation of genomic DNA from 50 to 400 µl of multiple sample types such as whole blood, saliva, buffy coat, buccal swabs, and more. The purified gDNA is ideal for a variety of downstream molecular biology applications.

Supplier: Thermo Fisher Scientific

KingFisher Duo-Ready DNA Ultra 2.0 prefilled plates are automation-ready plates prefilled with MagMAX DNA Multi-Sample Ultra 2.0 reagents suitably configured for KingFisher Duo and KingFisher Duo Prime purification systems. KingFisher Duo-Ready DNA Ultra 2.0 Prefilled Plates enable isolation of genomic DNA from 50 to 400 µl of multiple sample types such as whole blood, saliva, buffy coat, buccal swabs, and more. The purified gDNA is ideal for a variety of downstream molecular biology applications.