Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow
Supplier: Cytiva
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
Expand 1 Items
Amino acid modification reagents, sulfhydryl addition kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Sulfhydryl Addition Kit contains all necessary components to introduce free sulfhydryl groups into any primary amine-containing molecule, purify the modified molecule and quantify the added sulfhydryl groups.
Expand 1 Items
Yeast Geno-DNA Template™
Supplier: G-Biosciences
Yeast Geno-DNA Template™ isolates high quality genomic DNA from yeast. The kit is based on a two-step lysis of yeast cells, using LongLife™ Zymolyase® and LongLife™ Proteinase K, followed by the removal of proteins and other cellular impurities by precipitation and centrifugation.
Expand 1 Items
Affinity chromatography media, Heparin Sepharose™ 6 Fast Flow
Supplier: Cytiva
Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Expand 1 Items
Accessories for phosphopeptide enrichment and clean-up kit, Pierce™
Supplier: Thermo Fisher Scientific
Phosphopeptide enrichment spin tips with TiO2 resin, 96 tips, Pierce
Expand 1 Items
Accessories for Pierce Melon™ Gel Monoclonal IgG Purification Kit
Supplier: Thermo Fisher Scientific
Ascites conditioning reagent, Melon™
Expand 4 Items
Blue Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
Expand 1 Items
Ion exchange chromatography media, DEAE Sephacel™
Supplier: Cytiva
DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.
Expand 1 Items
Affinity chromatography media, MabSelect SuRe™
Supplier: Cytiva
MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.
Expand 2 Items
Classic magnetic IP/Co-IP kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Classic Magnetic IP/Co-IP Kit enables highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of antigens and protein-complexes using less than 10 µg of antibody and a magnetic separator.
Expand 1 Items
Affinity purification media, rProtein A Sepharose™ Fast Flow
Supplier: Cytiva
rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.
Expand 3 Items
SMART Digest™ Chymotrypsin Kits
Supplier: Thermo Fisher Scientific
SMART Digest™ Chymotrypsin Kits are designed to provide a complimentary digestion option to the SMART Digest™ Trypsin Kits which incorporate trypsin functionality. The Chymotrypsin enzyme functionality allows digestion of proteins at different points of the amino acid chain compared to trypsin. This allows for different/additional structural information to be obtained when trying to characterise a protein. This option can also be used if a protein does not contain the specific point where trypsin cuts the protein chain.
Expand 9 Items
Direct magnetic IP/Co-IP kit, Pierce™
Supplier: Thermo Fisher Scientific
The Direct Magnetic IP/Co-IP Kit uses NHS-chemistry to covalently immobilise IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation.
Expand 1 Items
SMART Digest™ proteinase K kits
Supplier: Thermo Fisher Scientific
Thermo Scientific™ SMART Digest™ Proteinase K Kits are designed to provide a simple to use, fast, highly reproducible and robust process for digestion of proteins in a high throughput format, meeting the needs of biopharmaceutical, bioanalytical and QA/QC environments. This version of the kit utilises Proteinase K, which cleaves the peptide bond adjacent to the carboxyl gropu of aliphatic and aromatic amino acids with blocked alpha amino groups.
Expand 9 Items
Accessories for protein A and G antibody purification
Supplier: G-Biosciences
IgG elution buffer
Expand 2 Items
Accessories for Anti-c-Myc Agarose, Pierce™
Supplier: Thermo Fisher Scientific
c-Myc peptide control, to elute c-Myc-tagged proteins from anti-c-Myc agarose, Pierce
Expand 1 Items
DNA isolation, Genomic DNA isolation kits, XIT™
Supplier: G-Biosciences
G-Biosciences offers a wide selection of genomic isolation kits that purify high quality genomic DNA from a variety of sources and for a wide array of applications. G-Biosciences XIT™ DNA kits produce protein-free, high quality DNA through the principle of cell lysis, protein digestion and precipitation and genomic DNA purification. No chloroform or phenol extraction is required. High quality DNA can be isolated from sample types including: animal tissues, cells, whole blood, bacteria, buccal cells, plant tissues, mouse tail, yeast and FFPE tissue. G-Biosciences XIT™ DNA kit procedures remove contaminants and enzyme inhibitors allowing the purified DNA to be ready for immediate use for all downstream analyses. The purified DNA from G-Biosciences XIT™ DNA kits have a A₂₆₀/A₂₈₀ ratio between 1,7 and 1,9 (with the exception of the buccal cells kit with a ratio between 1,8 and 2,0) and are up to 200 kb in size.
Expand 23 Items
Nucleic Acid Isolation Kits, illustra™ triplePrep Kit
Supplier: Cytiva
The illustra™ triplePrep Kit is designed for the rapid, simultaneous extraction and isolation of high yield genomic DNA (gDNA), total RNA, and total denatured proteins from undivided animal tissues and mammalian cells. The streamlined workflow reduces the overall number of steps, enabling the preparation of all three analytes in less than one hour.
Expand 1 Items
Affinity coupling media, Epoxy-Activated Sepharose™ 6B
Supplier: Cytiva
Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.
Expand 1 Items
MagBinder® Fit24 Nucleic Acid Purification System
Supplier: OMEGA BIO-TEK
Elevate your laboratory experience with the MagBinder® Fit24 Nucleic acid purification system. Efficiency, affordability, precision, and reliability, all in one powerful instrument.
Expand 1 Items
KingFisher™ Presto Purification System
Supplier: Thermo Fisher Scientific
Use the Thermo Scientific KingFisher Presto as part of an automated purification system with a liquid handler for plate filling in high-throughput laboratories.
Expand 1 Items
Duo-Ready DNA Ultra 2.0 prefilled plates, KingFisher™
Supplier: Thermo Fisher Scientific
KingFisher Duo-Ready DNA Ultra 2.0 prefilled plates are automation-ready plates prefilled with MagMAX DNA Multi-Sample Ultra 2.0 reagents suitably configured for KingFisher Duo and KingFisher Duo Prime purification systems. KingFisher Duo-Ready DNA Ultra 2.0 Prefilled Plates enable isolation of genomic DNA from 50 to 400 µl of multiple sample types such as whole blood, saliva, buffy coat, buccal swabs, and more. The purified gDNA is ideal for a variety of downstream molecular biology applications.
Expand 1 Items
Accessories for water purification systems BIOPURE 7/15
Supplier: ELGA LabWater
Ultra filtration cartridge, ENDOGARD, 5000 MWCO, removes particles as small as protein macromolecules
Expand 1 Items
Ready DNA Ultra 2.0 prefilled plates, for KingFisher Flex instruments, KingFisher™
Supplier: Thermo Fisher Scientific
KingFisher Ready DNA Ultra 2.0 Prefilled plates are automation-ready plates prefilled with MagMAX DNA Multi-Sample Ultra 2.0 reagents suitably configured for KingFisher Flex purification systems. KingFisher Ready DNA Ultra 2.0 prefilled plates enable isolation of genomic DNA from 50 to 400 µl of multiple sample types such as whole blood, saliva, buffy coat, buccal swabs, and more. The purified gDNA is ideal for a variety of downstream molecular biology applications.
Expand 1 Items
Nucleic acid purification system, MPure-12™, MP Biomedicals
Supplier: MP Biomedicals
MPure-12™ is a bench top automated system for rapid purification of nucleic acids from a wide variety of bio-specimens using magnetic bead separation technology. It has a uniquely designed magnetic bead processing chamber and is fully integrated with easy to use pre-packaged reagent kits.
Expand 1 Items
Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells. 1 * 1 mL
Supplier: Thermo Fisher Scientific
Pierce Anti-DYKDDDDK Affinity Resin provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins expressed in in vitro protein expression systems, bacteria, yeast, and mammalian cells. 1 * 1 mL
Expand 1 Items
Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells. 1 * 1 mL
Supplier: Thermo Fisher Scientific
Pierce Anti-DYKDDDDK Magnetic Agarose provides a fast, convenient method for purification and immunoprecipitation (IP) of DYKDDDDK-tagged proteins from in vitro protein expression systems, bacteria, yeast, and mammalian cells. 1 * 1 mL
Expand 1 Items
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. 1 * 400 l
Supplier: Aviva Systems Biology
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. 1 * 400 l
Expand 1 Items
ProteoAnalyzer Systems
Supplier: AGILENT
Automated SDS capillary electrophoresis system separates 12 protein samples in parallel.