"derivatisation reagent"

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Screw cap septum vials

Supplier: Thermo Fisher Scientific

These vials are ideal for sample collection and small derivatisation reactions for use with automated gas (GC) and liquid chromatography (LC), and for storage of reagents and standards under complete seal with instant syringe access. They are made of autoclavable borosilicate glass.

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NHS-Azide and NHS-phosphine reagents, Pierce™

Supplier: Thermo Fisher Scientific

The NHS-Azide and NHS-Phosphine Reagents are amine-reactive compounds for derivatising primary amines of proteins or amine-coated polymer surfaces. Once a protein or surface is azide- or phosphine-labelled, the two components are mixed for effective and stable conjugation. Phosphine groups react with azides via a Staudinger reaction to produce an aza-ylide intermediate that is trapped to form a stable, covalent amide bond. Because phosphines and azides are absent from biological systems, there is minimal background labelling of macromolecules found in cells or lysates.

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Carboxyl reactive biotinylation reagents, EZ-Link™

Supplier: Thermo Fisher Scientific

Carboxyl groups, in the form of carboxy termini, aspartate residues or glutamate residues, can be targeted for biotin labeling using amine-derivatised biotin molecules. This reaction is mediated by a class of crosslinkers known as carbodiimides and results in the formation of an amide bond. The reaction with EDC, the most common carbodiimide crosslinker, is generally performed in an MES buffer at pH 4,5 to 5 and requires just minutes to complete.