"derivatisation reagent"

Supplier: MACHEREY-NAGEL

HMDS is weak TMS donor. If used as sole reagent, it is slow and not very effective. After addition of catalytic quantities (e.g., 1 %) of TMCS or as a mixture with TMCS it is a fast and quantitative reagent for trimethylsilylation of organic compounds.

Supplier: MACHEREY-NAGEL

Methylation with TMSH is recommended for free acids, chlorophenoxycarboxylic acids, their salts and derivatives as well as for phenols and chlorophenols. Lipids or triglycerides can be converted to the corresponding fatty acid methyl esters (FAMEs) by a simple transesterification.

Supplier: MACHEREY-NAGEL

BSTFA is a powerful trimethylsilyl donor with approximately the same donor strength as the nonfluorinated analog BSA. Advantage of BSTFA over BSA: greater volatility of its reaction products (particularly useful for GC of some lower boiling TMS amino acids).

Supplier: MACHEREY-NAGEL

Acylation with fluorinated bisacylamides is recommended for alcohols, primary and secondary amines as well as for thiols under mild, neutral conditions.

Supplier: MACHEREY-NAGEL

MBDSTFA is a silylation reagent that donates a tert-butyldimethylsilyl group (TBDMS) for derivatizing active hydrogen atoms in hydroxyl, carboxyl and thiol groups, primary and secondary amines as well as amino acids. Fast reactions (typically 5–20 min) with high yields (> 96 %). By-products are neutral and volatile.

Supplier: Restek

Alkylation Derivatisation Reagents

Supplier: Apollo Scientific

Suitable reagent for the derivatisation of carboxylic acids for ECD and GCMS analysis.

Supplier: MACHEREY-NAGEL

Silyl-991 is recommended for silylation of fatty acid amides, hindered hydroxyls and other compounds which are difficult to silylate (e.g., secondary alcoholes and amines).

Supplier: Restek

Silylation is the most widely used derivatisation procedure for sample analysis by GC. In silylation, an active hydrogen is replaced by an alkylsilyl group such as trimethylsilyl (TMS) or tert-butyldimethylsilyl (tert-BDMS). Silyl derivatives are more volatile, less polar, and more thermally stable. As a result, GC separation is improved and detection is enhanced.

Supplier: SIGMA ALDRICH MICROSCOPY

4-Methoxybiphenyl has been used as a standard reagent whose fluorescence intensity is associated with the fluorescence characteristics of the products of derivatisation reaction for aryl halides with phenylboronic acid (PBA).

Supplier: Restek

Acylation reagents offer the same advantage available from silylation reagents: creating less polar, more volatile derivatives. In comparison to silylating reagents, the acylating reagents can more readily target highly polar multifunctional compounds, such as carbohydrates and amino acids. In addition, acylating reagents offer the distinct advantage of introducing electron-capturing groups, thus enhancing detectability during analysis.

Supplier: Abcam

Derivatisation reagent. Fluorescent label for amine modification and protein conjugation. Also used for labeling synthetic peptides for high-throughput detection.

Supplier: MACHEREY-NAGEL

Silyl-271 will derivatise all hydroxyl groups in any position. Useful in multiderivatisation schemes involving hydroxyl or amine groups.

Supplier: DWK Life Sciences

For storage of reagents and standards. Excellent for small derivatisation reactions. Wheaton 33® low extractable borosilicate glass is ideal for research studies where stability of glass is of prime importance.

Supplier: MP Biomedicals

Dansyl Chloride is a fluorogenic reagent for fluorescent N-terminal derivatisation of amino acids and peptides and detection by reverse phase HPLC. It is used in labeling proteins and amino acids.

Supplier: Abcam

with NO₂- under acidic conditions to form a detectable fluorescent naphthotriazole. The reaction proceeds at acidic pH at room temperature. Fluorescence is monitored following the addition of NaOH, which raises pH, resulting in lower background and increased sensitivity. Also used as a derivatisation-reagent for the determination of selenium at low detection limits.