buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
VWR®, Phosphate Buffered Saline (PBS), pH 7,4
Supplier: VWR Chemicals
Phosphate Buffered Saline (PBS) is a buffer solution containing sodium chloride, sodium phosphate and potassium chloride. PBS helps to maintain a constant pH, is isotonic and non toxic to cells, and provide cells with water and essential inorganic ions. This formulation does not contain calcium and magnesium.
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Buffer, TRIS solution pH 7,5 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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HDAC assay buffer II
Supplier: Enzo Life Sciences
50 mM Tris/Cl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/ml BSA.
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TM Buffer, 1X Tris-Magnesium sulphate, pH 7,5
Supplier: Rockland Immunochemicals
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 50 mM Tris HCl, 10 mM magnesium sulphate at pH 7,5 and was prepared using ultra pure reagents dissolved in pharmaceutical grade deionised water.
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Buffers, TE solutions 10X concentrates (TRIS-EDTA buffer)
Supplier: Rockland Immunochemicals
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
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Buffer, HEPES solution (1 mol/L)
Supplier: Cayman Chemical
HEPES buffer solution (1 mol/l)
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G-Biosciences Sodium Chloride-Sodium Citrate Buffer (20X)
Supplier: G-Biosciences
Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.
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Antigen Retrieval Buffer (100X Citrate Buffer), Abcam
Supplier: Abcam
Antigen retrieval 100X citrate buffer (pH 5.1 - 5.5) is a special solution that enables rehydration and antigen retrieval to be performed in formalin-fixed, paraffin-embedded tissue sections with one step. - Cited in over 25 publications - Contains citrate buffer, 1.5% 2-butoxyethanol, 1% hydrochloric acid - Optimized for citrate buffer-treated antibodies - Contains detergent for paraffin emulsification
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VWR® Sodium Acetate Buffer Solution, pH 5,2 (3 mol/L) for Biotechnology
Supplier: VWR Chemicals
Sodium acetate buffer, VWR®, 3 M, pH value: 5,2
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VWR®, Eluent buffers for amino acid analyzer
Supplier: VWR Chemicals
LC eluent B, Li
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Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0), Abcam
Supplier: Abcam
Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
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VWR® Tris Buffer Solution, pH 7,5 (1 mol/L), UltraPure Grade
Supplier: VWR Chemicals
TRIS buffer solution pH 7,5 (1 mol/l), VWR®
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Cell culture salts, salt solutions and buffers
Supplier: Biowest
These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.
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G-Biosciences TBE-Urea Sample Buffer
Supplier: G-Biosciences
Loading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands.
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Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0), Abcam
Supplier: Abcam
Antigen Retrieval Buffer (100X Tris Buffer, pH 10.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
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Extraction Buffer 5X PTR, Abcam
Supplier: Abcam
Extraction buffer 5X PTR is formulated for preparing cell or tissue extracts for use in immunoassays, with added phosphatase inhibitors and protease inhibitor aprotinin. - Cited in over 20 publications - Phosphatase and protease inhibitors – contains inhibitors to preserve sample integrity - Easy to use – dilute to 1X with distilled water before use, or use with extraction enhancer buffer 50X for enhanced extraction - Compatible with a variety of sample types



