buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations
Supplier: Mettler - Toledo
TISAB III solution for fluoride determinations
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10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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EDTA, 0.5M pH 8.0, Corning®
Supplier: Corning
EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.
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G-Biosciences Antigen Retrieval Solution, Citrate (pH 6,0) (50X)
Supplier: G-Biosciences
Solution developed to unmask antigens in paraffin embedded tissue sections.
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G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants
Supplier: G-Biosciences
ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.
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ISE sample conditioning buffers and solutions
Supplier: WTW
Ion strength adjustment solutions have multiple tasks: TISAB for fluoride measurements does not only adjust the pH value, by also decomplexes the fluoride-containing complex compounds. Other ion strength adjustment solutions ensure an adapted pH value as well as the addition of a suitable conducting salt to correctly capture the concentration.
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Buffer solutions
Supplier: REAGECON
Buffer solution, pH value: 4,00, ±0,01, Reference temperature: 25 °C, Plastic bottle
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pH buffer solutions, colour coded
Supplier: REAGECON
Colour coded pH buffer solutions for the calibration of pH meters.
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Hank's balanced salt solution, modified, MP Biomedicals
Supplier: MP Biomedicals
Cell culture buffer, Hank's balanced salt solution, modified (1X)
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pH electrode calibration and maintenance solutions kit
Supplier: WTW
Adapted to different electrode types for calibration and maintenance.
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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer), Sigma-Aldrich®
Supplier: Merck
Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)
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Hanks' Balanced Salts (Modified), 1X, without Magnesium and Calcium, MP Biomedicals
Supplier: MP Biomedicals
Used to minimise cell attachment or aggregation.
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Buffer, PBS solution (phosphate buffered saline) pH 7,4, Sigma-Aldrich®
Supplier: Merck
Washing buffer, PBS buffer solution (phosphate buffered saline) pH 7,4
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Hank's balanced salts, MP Biomedicals
Supplier: MP Biomedicals
Hanks' balanced salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.
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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®
Supplier: Merck
Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)
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Buffer, PBS solution 10X concentrate (phosphate buffered saline), Sigma-Aldrich®
Supplier: Merck
Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)