buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer solutions as per DIN 19266-01: 2000
Supplier: WTW
These standard buffer solutions (work reference buffer solutions) are widely used in precision applications.
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Low pH Phosphate SDS Sample Buffer
Supplier: EKSIGENT
SCIEX Low pH SDS phosphate sample buffer, 140 ml (40 mM Phosphate, pH 6,5, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a China Pharmacopeia compliant buffer.
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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free
Supplier: Thermo Fisher Scientific
Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid
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Sodium bicarbonate, Corning®
Supplier: Corning
Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment
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Dulbecco's phosphate buffered saline (DPBS), cell culture, MP Biomedicals
Supplier: MP Biomedicals
Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.
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Buffer Capsules
Supplier: Greisinger
Cost-effective alternative: Buffer capsules and working sets. With buffer capsules, you can produce a 100 ml buffer solution in the most cost-effective manner. A particular advantage of the capsules is that their storage life is several years longer than that of ready-to-use solutions.
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Blocking reagents, Blotto
Supplier: Rockland Immunochemicals
Contains immunoanalytical grade non-fat dry milk. Blocks non specific binding of proteins in various immunological assays. Ideal for use in western blotting and ELISA methods.
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Sodium acetate, Corning® 3M™
Supplier: Corning
Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.
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Phosphate Buffer Mixed pH 4,0
Supplier: CPACHEM
Shelf Life on Ship Date: 12 months - Store under normal laboratory conditions, at temperatures between 15 to 25 °C.
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TBS with Tween® (TBST), 20X solution, Molecular Biology Grade, ultrapure
Supplier: Thermo Fisher Scientific
TBS is used in Western blotting protocols.
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pH buffers, ready to use
Supplier: WTW
Every bottle has its own lot reference with the expiration date.
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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.
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Protexidase™, peroxidase protection buffer, Immuno™, MP Biomedicals
Supplier: MP Biomedicals
A peroxidase protective buffer which stabilises HRP conjugates. It has 500 ml of PBS, pH 7,2, containing 0,5% BSA, antimicrobial preservatives and proprietary stabilising compounds.
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EDTA disodium salt solution, 0,2% in phosphate buffered saline, MP Biomedicals
Supplier: MP Biomedicals
EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.
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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium
Supplier: MP Biomedicals
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)
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T-PER™ Tissue Protein Extraction Reagent
Supplier: Thermo Fisher Scientific
Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.