276358 Results for: "buffers and cell culture grade water hyclone"

Supplier: Biowest

Schneider’s Insect Medium was developed to support the growth of excised imaginal discs from the fruit fly, Drosophila melanogaster. When supplemented with 5 to 20% heat-inactivated foetal bovine serum Schneider's mediuam has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila spp. and several other dipterans.

Supplier: VWR Collection

Confocal dishes are suitable for fluorescent, confocal and phase-contrast microscopy experiments. These dishes combine the convenience of standard 35 mm, PS cell culture dishes with the optical benefits of glass. Dishes are available with Ø 20 mm glass centre, with or without a tissue culture-treated surface.

Supplier: Avantor

Avantor’s Water for Injection (WFI) quality water can help supplement existing media and buffer preparation processes by providing a reliable, high-quality alternative to in-house water production. Our water for injection quality water is manufactured under cGMP manufacturing guidelines using a multi-effect distillation process, ozone sparge and UV treatment. It is passed through a 0,2 µm filter prior to filling.

Supplier: Biowest

Ham's F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. It is frequently used with dialysed serum, hormones, selenium and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat and has proved to be an excellent cloning medium for the culture of myeloma and hybrid cells.

Supplier: Biowest

Basal Medium Eagle is principally used for monolayer diploid or primary cell cultures. It is the simplest of the basal media with all essential components for growth. BME ideally supports cell lines such as HeLa, L-cells and primary mammalian fibroblasts. BME has also successfully been used for the growth of human transformed cell lines (particularly WI-38 and MRC-5).

Supplier: VWR Chemicals

Lymphocyte separation media is a density gradient medium for the isolation of mononuclear cells from peripheral blood, cord blood and bone marrow.

Supplier: Biowest

Minimum Essential Medium (MEM) with Earle's Balanced Salts is a modification of Eagle's earlier medium, Basal Medium Eagle (BME), containing higher concentrations of the essential nutrients. These media have demonstrated the ability to support a variety of normal and transformed cells in culture and contain Earle's Balanced Salts, which make them suitable for use in atmospheres charged with CO₂ gas.

Supplier: Greiner Bio-One

PS, cell culture-treated, sterile.

Supplier: MP Biomedicals

Rich medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

Supplier: Biowest

Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Supplier: HyClone products (Cytiva)

DMEM Low Glucose was the original modification of Basal Medium Eagle (BME) used to grow mouse embryonic cells. DMEM, Low Glucose is suitable for the culturing of many immortalised cell lines from humans, monkeys, hamsters, rats, mice, chicken and fish. DMEM contains four times more amino acids, vitamins and other supplementary components than Basal Medium Eagle (BME), the base media from which DMEM was derived. As DMEM contains no growth-promoting agents or proteins, supplementation with FBS is recommended for optimal performance.

Supplier: Biowest

Glasgow MEM (GMEM) was originally formulated for growing BHK 21 cells. The original formulation is an Eagle's Basal Medium modified to contain two times the normal concentration of amino acids and vitamins. It is intended for use with adherent cell lines.

Supplier: VWR Collection

VWR® cell and tissue culture dishes are available with a choice of surface treatments.

Supplier: Corning

TC-treated gridded culture dishes.

Supplier: VWR Chemicals

VWR® Bovine Serum Albumin (BSA) is used in many applications as a stabilisation agent. Albumin is used to solubilise lipids, and is also used as a blocking agent in Western blots or ELISA applications. It is commonly used in cell culture protocols mainly for protein supplementation. It can also be added to viral transport media for stabilisation.

Supplier: MP Biomedicals

Basal Medium Eagle is suited for the isolation and cultivation of non-fastidious and fastidious microorganisms from a variety of clinical and non-clinical specimens. It can also be used as a selective isolation medium by adding antimicrobial agents.

Supplier: MP Biomedicals

Minimum Essential Medium (MEM) is a general use medium ideal for the growth and maintenance of a wide range of mammalian cell types. MEM with Earle's salts and L-glutamine has demonstrated the ability to support a variety of normal and transformed cells in culture and contains Earle’s balanced salts, which makes it suitable for use in atmospheres charged with CO₂ gas.

Supplier: Greiner Bio-One

PS, coated with rat tail collagen type I. Collagen type I is an extracellular matrix protein, which in vivo influences adhesion, migration and proliferation, amongst other processes. Many otherwise difficult-to-cultivate cells adhere to collagen type I and show positive growth behaviour. For certain cell lines collagen type I also has an influence on differentiation and morphology.

Supplier: STEMCELL Technologies

HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.

Supplier: PROVITRO

Skeletal Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human skeletal muscle cells (HSKMC). Delivered as a basal medium, it is suitable for culturing Provitro HSKMC upon the addition of supplement mix components.

Supplier: Biowest

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukaemia cells.

Supplier: Greiner Bio-One

PS, cell culture-treated, sterile. Available with a variety of different dimensions and growth areas. The 57 cm² and 143 cm² dishes have an extra high profile, with a height of 20 mm. All dishes are certified free of RNase, DNase and human DNA, are non pyrogenic and non cytotoxic.

Supplier: VWR Chemicals

VWR® penicillin-streptomycin solution (100X) is a large spectrum antibiotic designed to control and destroy gram-negative and gram-positive bacteria.

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

Supplier: Greiner Bio-One

These sterile, PS cell culture dishes have a cell-repellent surface to effectively inhibit cell attachment. Achieved through a chemical polymer modification, the surface does not degrade or leach under common cell culture conditions, rendering an ideal substrate for native cell culture experiments.

Supplier: PROVITRO

Endothelial cell proliferation medium is a sterile liquid culture medium for culturing endothelial cells. The medium is delivered as a basal medium and is suitable for culturing human endothelial cells after adding the supplement mix components.

Supplier: PROVITRO

Smooth Muscle Cell Growth Medium is a sterile liquid culture medium designed for cultivating human smooth muscle cells (HSMC). Provided in the form of a basal medium, it is ideal for culturing HSMC upon the addition of supplement mix components.