275704 Results for: "buffers and cell culture grade water hyclone"

Supplier: Biowest

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

Supplier: HyClone products (Cytiva)

Dissociation reagents, such as trypsin, are used to detach and disaggregate adherent cells from the surface or substrate to which they are attached, as well as for dissociating tissues for primary cell culture.

Supplier: VWR Chemicals

Water, AnalaR NORMAPUR® ISO 3696 Grade 3 analytical reagent

Supplier: VWR Chemicals

Water, nuclease-free, molecular biology grade (RNase, DNase and protease tested).

Supplier: G-Biosciences

Molecular Grade Water is ultrapure water that certified RNase, DNase and Protease free, is suitable for use in molecular biology applications, which demand a high quality of water and assurance that the water is free from nucleases and protease contamination. No toxic agents, such as DEPC, are used in the manufacturing of Molecular Grade Water, eliminating DEPC interference of enzymatic reactions.

Supplier: Biowest

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

Supplier: VWR Chemicals

VWR® PFAS grade LC-MS solvents undergo a rigorous use test to ensure suitability for analysis of poly- and perfluoroalkyl substances resulting in an ultra low background of PFAS analytes. Using complementary methods a broad range of PFAS analytes are quantified to their detection limits by LC-MS.

Supplier: VWR Chemicals

Molecular Biology grade water, nuclease-free is suitable for use in polymerase chain reaction and other molecular biology techniques requiring sterile nuclease-free conditions, such as in processing DNA or RNA (i.e., PCR, cDNA synthesis, qPCR or NGS Library).

Supplier: HyClone products (Cytiva)

SG-200 solution is a stable dipeptide of L-alanyl L-glutamine.

Supplier: Apollo Scientific

Modified version of product number PMM519, contains 30 g/L D-Sucrose. Plant Tissue Culture Tested. Soluble in water.

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 50 mM Tris HCl, 10 mM magnesium sulphate at pH 7,5 and was prepared using ultra pure reagents dissolved in pharmaceutical grade deionised water.

Supplier: Enzo Life Sciences

Sterile, double distilled endotoxin-free water for use with TLRgrade™ or endotoxin-free grade reagents.

Supplier: HyClone products (Cytiva)

HyClone™ DMEM/F12 is an equal parts blend of DMEM and Ham’s F12 media that is widely used for culturing many different mammalian cells including MDCK, Glial cells, fibroblasts, and endothelial cells. This formulation combines the high concentration of glucose, amino acids and vitamins from DMEM with the wide variety of other components available in Ham’s F-12.

Supplier: MP Biomedicals

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.

Supplier: VWR Chemicals

Water, HiPerSolv CHROMANORM®, super gradient grade for HPLC, suitable for UPLC/UHPLC instruments

Supplier: HyClone products (Cytiva)

HyClone Leibovitz L-15 Medium is manufactured using ISO 9001:2000-certified processes

Supplier: VWR Chemicals

Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO₂ gas.

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7,2 to 7,6).

Supplier: VWR Collection

VWR® 3-D scaffold cell culture dishes, made of polystyrene (GPPS) with polystyrene polymer scaffold, are able to simulate the three-dimensional structure of cells in animals and the human body to maximum effect, providing an ideal environment for cell interaction, maximising culture area and improving yield.

Supplier: MP Biomedicals

Deionized water treated with 0.001% diethylpyrocarbonate (DEPC). Filtered through 0.2 micron filter and autoclaved to yield a sterile solution completely free of detectable DNase (both exo and endo) and RNase.

Supplier: VWR Chemicals

RPMI are general purpose enriched media. Originally formulated for suspension cultures and monolayer cultures of human leukemic cells.

Supplier: VWR Chemicals

Ham's F12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. Also used in a clonal toxicity assay using CHO cells. It is frequently used with supplements for serum-free cultures to support the growth of cells of rodent origin, or as a cloning medium for the culture of myeloma and hybrid cells.

Supplier: VWR Chemicals

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: VWR Chemicals

Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.

Supplier: VWR Collection

Confocal dishes are suitable for fluorescent, confocal and phase-contrast microscopy experiments. These dishes combine the convenience of standard 35 mm, PS cell culture dishes with the optical benefits of glass. Dishes are available with Ø 20 mm glass centre, with or without a tissue culture-treated surface.

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

Supplier: Biowest

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: VWR Chemicals

Schneider's Drosophila medium promotes rapid growth of both primary and existing cell cultures derived from Drosophila spp and several other dipterans when supplemented with 5 to 20% heat-inactivated foetal bovine serum.

Supplier: Avantor

Avantor’s Water for Injection (WFI) quality water can help supplement existing media and buffer preparation processes by providing a reliable, high-quality alternative to in-house water production. Our water for injection quality water is manufactured under cGMP manufacturing guidelines using a multi-effect distillation process, ozone sparge and UV treatment. It is passed through a 0,2 µm filter prior to filling.