326 Results for: "VARLP080520VARI00001"

Supplier: SIGMA ALDRICH MICROSCOPY

5-Fluorouracil has been used to induce apoptosis in cells. It has been used as a chemosensitising agent. A potent antitumor agent that affects pyrimidine synthesis by inhibiting thymidylate synthetase, thus depleting intracellular dTTP pools. It is metabolized to ribonucleotides and deoxyribonucleotides, which can be incorporated into RNA and DNA. Treatment of cells with 5-FU leads to an accumulation of cells in S-phase and has been shown to induce p53 dependent apoptosis.

Supplier: SIGMA ALDRICH MICROSCOPY

The release of phosphate or pyrophosphate in an enzymatic reaction can be detected by malachite green and is measured spectrometrically at 660 nm.

Supplier: SIGMA ALDRICH MICROSCOPY

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in gram staining.

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Wright stain, a modified romanowsky stain, is intended for differential staining of the cellular elements of blood. It comprises polychrome methylene blue and eosin B. Upon staining blood films with wright stain, the white blood cell nucleus and cytoplasm display characteristic blue or pink hues.

Supplier: SIGMA ALDRICH MICROSCOPY

Chromotrope 2R has been used in the Gomori trichrome staining in osteochondral tissues. It has also been used for the staining of eosinophils in lung sections. Chromotrope 2R is used for the staining of microsporidia in body fluids and stool samples. It is used in modified Gomori′s trichrome staining. Chromotrope 2R has high affinity for phospholipids and is used for the staining of mitochondrial and endoplasmic reticulum membrane in histological samples.

Supplier: SIGMA ALDRICH MICROSCOPY

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

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Membrane impermeant, phenanthridinium intercalator dye excited by mercury or xenon-arc lamps or argon-ion laser. Suitable for use in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry. Used as a nuclear counterstain.

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1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) is a photochemically stable electron-transport mediator between NAD(P)H and tetrazolium dyes. The reduced form of MPMS converts tetrazolium salt to purple precipitate formazan. It is a very stable and non-enzymic electron carrier that mediates electron transfer by dehydrogenases in vitro to various electron acceptors such as tetrazolium dyes or the electrode of an enzymic electric cell.

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Phloroglucinol (phlo) is a phenol derivative that shows a cytoprotective effect from oxidative damage by enhancing the activity of cellular catalase. It can also be used to prepare synthetic analogs of A-type proanthocyanidins (PACs) such as 2,8-dioxabicyclo nonane derivatives by reacting with the corresponding flavylium salts. Phlo can react with benzaldehyde derivatives to form phloroglucinol-based microporous polymeric organic frameworks (phlo-POF) with potential applications in ion exchange and gas adsorption. Phloroglucinol is an antispasmodic and a derivative of phenol.

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Direct Red 80 (C.I. 35780) is an extremely hydrophilic, very large tetrakisazo hexasulfonated anionic dye that has a large, planar aromatic system.It is also known as Sirius red F3B, chlorantine fast red 7BLN or 5BRL, durazol brilliant red B, pontamine fast red 7BNL, and sirius fast red.

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Fast green FCF, Sigma-Aldrich®

Supplier: VWR Chemicals

X-Gal (5-bromo-4-chloro-3-indolyl-ß-D-galactopyranoside) ≥99% (by enzymatic purity), Ultra Pure Grade

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Ponceau S (C.l. 27195) is an anionic, very hydrophilic disazo dye. It is also known as fast ponceau 2B. Ponceau S is widely used to stain proteins on gel electropherograms. It is a suitable stain for proteins adsorbed onto nitrocellulose membranes. It also has applications in botanical staining manuals as a stain for sieve plate elements.

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Methyl Green is a triphenylmethane dye which is mainly used in combination with pyronin. It is suitable for the detection of DNA and RNA. Used in the Methyl Green-Pyronin methods to demonstrate DNA and RNA in tissue sections.

Supplier: SIGMA ALDRICH MICROSCOPY

Patent blue V sodium salt has been used as a stain in pharmacology. It is mainly used as a food color.

Supplier: SIGMA ALDRICH MICROSCOPY

Readily polymerizes in aqueous solution, forming a stable free radical that inhibits protein-nucleic acid interactions. It is a potent inhibitor of ribonuclease and topoisomerase II by preventing the binding of the nucleic acid to the enzyme. It stimulates tyrosine phosphorylation processes including the Jak2/STAT5 pathway in NB2 lymphoma cells, ErbB4 in neuroblastoma cells, and MAP kinases, Shc proteins, phosphatidylinositide 3-kinase and phospholipase Cγ in PC12 cells. Inhibits apoptosis. It prevents down-regulation of Ca²⁺ -impermeable GluR2 receptors and inhibits calpain, a Ca²⁺ -activated protease that is activated during apoptosis.

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3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4',4"-disulfonic acid sodium salt is also known as ferrozine.

Supplier: SIGMA ALDRICH MICROSCOPY

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The following are two common stain formulations: 0.1% Ponceau S (w/v) in 5% acetic acid 2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid.

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Tetrabromophenol Blue (TBPB) belongs to the group of sulfonephthalein acid-base indicators. It is employed in the potentiometric titration of the halogens with Hg₂(NO₃)₂. In addition, it has been used as a dye indicator in urine dipstick test to detect proteinuria. In this assay, with increasing concentration of urinary protein, TBPB sequentially changes colour from pale green to blue. Binding of TBPB to proteins is dependent on pH.

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Erythrosin extra bluish is a brown color powder, that is soluble in ethanol and water. Its pH ranges from 2,5 to 4. It is used in color filters, light emitting diodes, cosmetics, hair dyes and more. It has several biological applications like detecting gene expression, treating age related macular degeneration, diabetes, obesity and more.

Supplier: SIGMA ALDRICH MICROSCOPY

Mixture of crystal violet [C.I.No. 42555] and methyl violet [C.I.No. 42535]. Dye employed for preparing Gram stain.

Supplier: SIGMA ALDRICH MICROSCOPY

Sunset yellow FCF is a food dye, which is is substantially used in various food preparations. It is a orange-red coloured crystal, that is soluble in water and ethanol. It is used in treating bone metabolic diseases, dermatological disorders, respiratory illness etc. It can alter the functional responses of splenocytes at non-cytotoxic dose. It promotes immune suppression. Relative expression of surface receptors in T and B cells can be altered by sunset yellow FCF.

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl Blue is a triaminotriphenylmethane dye, widely used in paper, textile and leather production.

Supplier: SIGMA ALDRICH MICROSCOPY

Acriflavine also interacts with HIF-1α (hypoxia-inducible factor 1 α) and HIF-2α, and thereby suppresses dimer formation of HIF-1 and transcriptional function. It also has negative effect on tumor growth and vascularization. Acriflavine also exhibits trypanocidal, antibacterial and antiviral activities.

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Light green SF yellowish, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt is a chelating agent for ferrous iron. It is also known as Ferrozine.

Supplier: SIGMA ALDRICH MICROSCOPY

Silver nitrate along with iodine are among several inorganic substances used to induce colour in microscopical preparations. Silver nitrate as a biological stain exploits the strong colors of colloidal metallic silver. Silver nitrate is an important component of the silver staining that is used for the detection of proteins separated by polyacrylamide gel electrophoresis. Silver nitrate is a component of the silver stain kit. It is required for image development in diamine as well as non-diamine silver staining.

Supplier: SIGMA ALDRICH MICROSCOPY

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Supplier: SIGMA ALDRICH MICROSCOPY

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromocresol purple sodium salt, Sigma-Aldrich®