"SureVector Cloning Kit"
PCR cloning kit, CloneJET
Supplier: Thermo Fisher Scientific
The CloneJET PCR cloning kit contains a ready-to-use positive selection cloning vector pJET1.2/blunt. The vector contains a lethal restriction enzyme gene that is disrupted by ligation of a DNA insert into the cloning site. As a result, only bacterial cells with recombinant plasmids are able to form colonies. Recircularised pJET1.2/blunt vector molecules lacking an insert express a lethal restriction enzyme, which kills the host E. coli cell after transformation. This positive selection drastically accelerates the process of colony screening and eliminates additional costs required for blue/white selection.
Expand 2 Items
T4 DNA ligase
Supplier: Thermo Fisher Scientific
This T4 DNA ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.
Expand 1 Items
Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: Thermo Fisher Scientific
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Expand 1 Items
VWR® Ultra Pure Grade, Sm buffer
Supplier: VWR Chemicals
Cloning Buffer, SM BUFFER
Expand 1 Items
VWR® Sodium Acetate Buffer 3,0 M Solution, pH 5,2
Supplier: VWR Chemicals
With diethyl pyrocarbonate (DEPC).
Expand 1 Items
Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)
Supplier: Thermo Fisher Scientific
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
Expand 1 Items
VWR®, EDTA buffer solution pH 8,0 (0,5 mol/L) for biotechnology
Supplier: VWR Chemicals
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l) for biotechnology
Expand 1 Items
EDTA Buffer, pH 8,5, 10X, Antigen Retriever
Supplier: SIGMA ALDRICH MICROSCOPY
Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
Expand 1 Items
Buffer, TRIS solution pH 7 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 7 (1 mol/L)
Expand 3 Items
10X Tris-EDTA buffer pH 9,0
Supplier: Novus Biologicals
10X Tris-EDTA buffer pH 9.0.
Expand 1 Items
VWR®, Tris buffer solution, pH 9 (1 mol/L), ultrapure
Supplier: VWR Chemicals
Cloning Buffer, TRIS buffer solution pH 9 (1 mol/l)
Expand 1 Items
VWR®, TE (Tris-EDTA) buffer, pH 8,0 for biotechnology
Supplier: VWR Chemicals
Ready to use Tris-EDTA buffer.
Expand 2 Items
EDTA, 0.5 M solution, pH 8.0, for biotechnology
Supplier: VWR Chemicals
Cloning Buffer, EDTA, 0,5 M solution
Expand 2 Items
Buffer, TRIS solution pH 8 (1 mol/L)
Supplier: PanReac AppliChem
Cloning Buffer, TRIS buffer solution (1 mol/l)
Expand 1 Items
Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Expand 3 Items
Buffer, sodium acetate buffer solution pH 5,2 (3 mol/l)
Supplier: G-Biosciences
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)



