"Staining Solutions"

Supplier: VWR Collection

peqGREEN dye is a safe alternative to ethidium bromide for staining of dsDNA, ssDNA and RNA.

Supplier: Biotium

Ethidium Homodimer, first developed by Dr. Le Pecq and his colleagues, is a high-affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes.

Supplier: G-Biosciences

A single step stain for the rapid detection of proteins resolved by PAGE. The stain is based on the interaction of zinc ions with polyacrylamide and proteins.

Supplier: MP Biomedicals

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxicity and proliferation assays.
Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system.

Supplier: Abcam

Ponceau S Solution (ab270042) is a ready-to-use reversible stain which allows you to check the efficiency of electrophoretic transfer of SDS-denatured proteins to nitrocellulose and PVDF membranes.

Supplier: SIGMA ALDRICH MICROSCOPY

Kit component in the silver stain kit.

Supplier: SIGMA ALDRICH MICROSCOPY

Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time. 4 g/L certified hematoxylin.

Supplier: SIGMA ALDRICH MICROSCOPY

Histo/Zyme is a stabilized proteolytic enzyme in buffered solution, used to unmask immunoreactive sites altered by fixation and/or the embedding process. Treatment with a proteolytic enzyme in immunohistochemistry (IHC) is required to digest excess linkages and expose the antigen. The use of Histo/Zyme results in superior staining over traditionally used enzyme pretreatments, such as pepsin and trypsin. Moreover, Histo/Zyme requires only a brief incubation at room temperature. Performing antigen recovery with Histo/Zyme will allow you to dilute the primary antibody 5 to 10 fold further and achieve optimal staining.

Supplier: SIGMA ALDRICH MICROSCOPY

Thymol is generally added in the Hematoxylin and Eosin staining solution to prevent mould growth. It is also suitable for use in antimicrobial screening by the disk diffusion method. It has been used as a test compound in antifungal assay to assess the susceptibility of various fungal isolates.

Supplier: VWR Chemicals

Lugol's iodine solution stabilised

Supplier: Thermo Fisher Scientific

Sodium acetate buffer solution pH 5,2 is been widely used in for precipitation of nucleic acids and preparation of gel stains for protein gel electrophoresis.

Supplier: SIGMA ALDRICH MICROSCOPY

To be used in conjunction with HT107. Recommended in lengthy or acidic stain procedures. Resists decolorization better than aluminum based hematoxylin formulations.

Supplier: Biotium

PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

Supplier: CELLPATH

Ready-to-use solution of alcohol and carbowax, which gives similar fixation results and staining.

Supplier: SIGMA ALDRICH MICROSCOPY

Orange G (certified), 0.3% and phosphotungstic acid, 0.015%, in denatured alcohol

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for use with Carbol Fuchsin (HT80-1) in Sigma Acid Fast Stain, Procedure HT80. Acts as both a counterstain and a differentiator.