"Staining Solutions"
ADVASEP™-7, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
ADVASEP™-7 has been used in washing solution to remove excess dyes, FM4-64FX and FM1-43, in stained cells.
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Ponceau Xylidine ≥60 % (dye content), Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Ponceau Xylidine has been used as part of the Goldner’s trichome stain to stain thin sections. It has also been used to prepare Fuchsin-Ponceau solution to stain an amyotrophic lateral sclerosis tissue-engineered
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Ethidium bromide 0,625 mg/l in aqueous solution, Biotechnology Grade
Supplier: VWR Chemicals
Ethidium bromide solution is a fluorescent stain for visualising nucleic acids in solution or in electrophoresis gels. The solution is provided at a concentration of 0,625 mg/ml. A final working concentration of 0,5 g/ml is easily obtained by adding one drop of solution to 50 ml of molten agarose, running buffer or staining buffer. Ethidium bromide solution in dropper bottles may be stored at room temperature for up to one year.
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Iron hematoxylin, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Recommended in lengthy or acidic stain procedures. Resists decolorisation better than aluminum based hematoxylin formulations. Weigert's iron hematoxylin solution set consists of HT107 Part A and HT109 Part B.
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Coomassie Brilliant Blue Destaining Solution, G-Biosciences
Supplier: G-Biosciences
After Coomassie Brilliant Blue staining process, the band intensity may be further enhanced by de-staining the stained gel in our CBB De-Staining Solution.
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Methylene blue concentrated solution (Ehrlich) for microscopy, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Solution, for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution (Methylene blue stain; Ehrlich's reagent III; Loeffler's Methylene Blue)
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Helixyte™ Gold Nucleic Acid Gel Stain *10000X DMSO Solution* (1 ml)
Supplier: AAT Bioquest
Helixyte™ Gold is manufactured by AAT Bioquest, and it has the same chemical structure of SYBR® Gold (SYBR® is the trademark of ThermoFisher).
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Azure II, powder, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Azure II has been used in the Richardson′s stock solution for the staining of lysosomal glycogen. It has also been used in the periodic acid schiff staining procedure for the staining of glycogen, nuclei and cytoplasm.
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Methylene blue solution for microbiology, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Ehrlich’s reagent III, Loeffler’s Methylene Blue. Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).
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Alcian blue solution, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.
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Wright's stain used as a blood stain, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.
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Oil red O 0.5% in propylene glycol, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.
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Coomassie brilliant blue G-250 solution electrophoresis gel stain, ready-to-use
Supplier: G-Biosciences
G-Biosciences' Coomassie Brilliant Blue (CBB) G-250 and R-250 Stain are based on a colloidal Coomassie stain. CBB G-250 and R-250 stain proteins with high band visibility. The staining of gels with CBB G-250 and R-250 allows the examination of protein bands even during the staining process.
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Crystal violet 1% in aqueous solution, Sigma-Aldrich®
Supplier: SIGMA ALDRICH MICROSCOPY
Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.
Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.
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Alcian blue
Supplier: Abcam
Cationic dye. Forms insoluble complexes with glycosaminoglycans in solution. Stains interphase nuclei, especially mitotic figures.
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Silver nitrate ≥99% ACS
Supplier: VWR Chemicals
Staining protein after electrophoretic separation. Also used to determine chloride ions in solution.