"Staining Solutions"

Supplier: SIGMA ALDRICH MICROSCOPY

To be used with SIAL 69571 Ren HY 956 (Hardener for Epoxy Embedding Medium)

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil has been used in epifluorescent microscopy and cell identification.

Supplier: VWR Chemicals

Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.

Supplier: SIGMA ALDRICH MICROSCOPY

ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: Abcam

Flow Cytometry Fixation & Permeabilization Kit ab314689 contains optimally formulated buffers for fixation and permeabilization of cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry.

Supplier: SIGMA ALDRICH MICROSCOPY

Epoxy embedding medium is generally used for biological tissues during study by electron microscopy. Usually, it consists of three components; Epon 812 resin, an anhydride hardener and an accelerator

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.

Supplier: SIGMA ALDRICH MICROSCOPY

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

Supplier: MP Biomedicals

Alcian Blue 8GX is a cationic dye used to quantitatively determine glycosaminoglycans by forming insoluble complexes in solution.

Supplier: VWR Chemicals

HistoChoice® clearing agent is a much less hazardous replacement for xylene/toluene.

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.

Supplier: VWR Chemicals

Fixative, combining the coagulant qualities of alcohol, to enable a better fixation of nuclear and cytoplasmic elements. The erythrocytes are lysed and, therefore, do not obscure observation of the elements of interest. This fixative can be used according to the Saccomanno method.

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable for use with large and small mineralized (undecalcified) bone sections. Yields clear, hard blocks for cutting sections. Not water-soluble.