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"Staining Solutions"

 
 
Carmine alum solution for dye

Carmine alum solution for dye

Supplier: Stemcell Technologies

Dye for staining mouse mammary gland whole mounts.

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ABTS® Peroxidase Substrate Kit, 1-Component Microwell

Supplier: SERACARE LIFE SCIENCES

KPL ABTS 1-component microwell peroxidase substrate develops a blue-green product when reacted with horseradish peroxidase labeled conjugates in microwell plates or tubes. The expected appearance of
the solution is colorless to light green. Acceptable background absorbance of unreacted substrate is ≤0,025, as measured at 405 nm. KPL ABTS 1-component microwell peroxidase substrate is not suitable for membrane or immunohistochemical staining.

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Wright's stain for microscopical staining, Sigma-Aldrich®

Supplier: Merck

Wright's stain for microscopical staining, Sigma-Aldrich®

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Methyl Green-Pyronin Y solution, Sigma-Aldrich®

Methyl Green-Pyronin Y solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Pyronin stains the cytoplasm of plasma cells and most nucleoli red.

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Osteo-Bed Bone Embedding Solvent

Supplier: SIGMA ALDRICH MICROSCOPY

For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

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May Grunwald's eosin-methylene blue solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

May Grunwald's eosin-methylene blue solution, Sigma-Aldrich®

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Experimentation kits, bacterial gram staining

Experimentation kits, bacterial gram staining

Supplier: G-Biosciences

The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.

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May Grunwald's eosin-methylene blue solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

May Grunwald's eosin-methylene blue solution, Sigma-Aldrich®

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Aqueous mounting medium, Fluoromount™, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.

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Ethidium bromide Greenbag™ disposal kit, MP Biomedicals

Supplier: MP Biomedicals

The EtBr Greenbag™ Disposal Kit allows rapid and trouble-free concentration of ethdium bromide from large volumes of solutions into a small 'teabag' containing activated carbon. The Greenbag™ is then conveniently disposed of along with other solid hazardous wastes.

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3D Clear tissue clearing reagents and buffers, Corning®

3D Clear tissue clearing reagents and buffers, Corning®

Supplier: Corning

In our Starter Kit we have combined 30 ml of Corning 3D Clear with the four buffer solutions required to process 3D tissue cultures for immunolabelling. All you need to do is add your immunolabels and nuclear stains. This kit comes with enough material to process three 96-well plates of 3D cell culture models.

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May Grunwald's eosin-methylene blue solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

May Grunwald's eosin-methylene blue solution, Sigma-Aldrich®

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Methylene blue solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm.

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Automated cell counter, ADAM-MC2

Automated cell counter, ADAM-MC2

Supplier: NANOENTEK

ADAM-MC2 is an automated fluorescence cell counter that performs cell counting and viability measurements at highest accuracy capable of replacing complex flow cytometry workflows. Combined with the disposable micro-fluidic Accuchip, operation is extremely simple and cost-effective. Utilising the PI (Propidium Iodide) nucleus staining method, most advanced LED optics and CMOS detection technology, ADAM-MC2 is the perfect solution when working with aggregated, irregular shaped or challenging cells like CAR-T cells, CAR-NK cells or adipose-derived stem cells.

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Anti-BrdU Mouse Monoclonal Antibody (FITC) [clone: PRB-1]

Anti-BrdU Mouse Monoclonal Antibody (FITC) [clone: PRB-1]

Supplier: Tonbo Biosciences

The incorporation of BrdU into newly synthesized DNA by actively cycling cells is one method for measuring the changing amount of cellular DNA during cell proliferation through each of the cell cycle phases. As a thymidine analog, BrdU is preferentially incorporated into newly replicated DNA which can then be subsequently labeled and analyzed to determine relative DNA content and cell cycle position. Incorporation of BrdU is most commonly detected using anti-BrdU antibodies. A BrdU solution is provided for exposure of actively cycling cells to incorporate BrdU. The EZ-BrdU Kit employs an acid denaturation step. The mild acid method used helps reduce damage to other cellular proteins. After the denaturation step, cells are stained with a FITC anti-BrdU antibody and total DNA is counterstained with a PI/RNase A solution. Two color flow cytometry can then be used to analyze cells that have incorporated BrdU (proliferating cells) in terms of their cell cycle position (G0/1, S, or G2/M phase). The EZ-BrdU Kit is shipped in one container and consists of two packages. Upon arrival one should be stored at 2-8°C and the other at -20°C.

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Malachite green oxalate solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Staining of bacterial spores according to Schaeffer and Fulton; Pappenheim stain when combined with Gram′s stain

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