"Staining Solutions"
Pierce™ ABC Staining Kits
Supplier: Thermo Fisher Scientific
ABC Staining Kits include reagents for the avidin-biotin complex (ABC) technique, a highly sensitive method for immunohistochemical detection with biotinylated secondary antibodies.
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FluoroQuest™ Fluorescence Signal Enhancing Solution (5 ml)
Supplier: AAT Bioquest
When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50 to 70%.
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VWR®, Destaining bags
Supplier: VWR Chemicals
Destaining bags are designed to safely remove ethidium bromide from solution during overnight treatment. Each bag extracts up to 5 mg of ethidium bromide from solution, thus minimising the exposure of research personnel to this toxic material. The special absorbent mixture retains dye molecules in a convenient bag for removal and incineration.
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5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP (p-toluidine salt), X-Phosphate) substrate ≥99%
Supplier: Thermo Fisher Scientific
Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.
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Trypan blue 0,4% in PBS
Supplier: HyClone products (Cytiva)
Trypan Blue solution, 0,4%, is routinely used as a cell stain to assess cell viability using the dye exclusion test.
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Trypan blue 4 g/l in aqueous solution cell culture reagent
Supplier: VWR Chemicals
A pre-mixed dye solution used in cell culture applications to determine cell viability. A researcher can remove a sample of cells from culture and combine in a 1:1 ratio with the Trypan Blue solution. Under the microscope, dead cells will appear a blue color and viable cells will appear clear and translucent. Using a hemacytometer (gridded microscope slide), a researcher can quantify the percentage of dead cells within a population. Stained cells are ready for counting within five minutes.
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4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride) 10 mM in water ≥95%
Supplier: AAT Bioquest
DAPI is a fluorescent stain that binds strongly to DNA.
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Lugol's iodine solution, Sigma-Aldrich®
Supplier: Merck
Lugol's iodine solution, Sigma-Aldrich®
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Giemsa's stain for clinical diagnosis
Supplier: PanReac AppliChem
Azur-eosin-methylene blue solution according to Giemsa (slow) for clinical diagnosis.
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Agarose gel loading dye 6X for electrophoresis, glycerol based, no xylene Cyanol FF
Supplier: Thermo Fisher Scientific
6X DNA Loading Buffer is a pre-mixed solution used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels.
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Papanicolaou's Orange G solution (OG 6)
Supplier: CLIN TECH
Papanicolaou's Orange G solution (OG 6)
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APO-BRDU™ kit, TUNEL Assay
Supplier: Tonbo Biosciences
The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
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Wright's stain for microscopical staining
Supplier: Thermo Fisher Scientific
Wright's stain for microscopical staining
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Wright's stain for microscopical staining
Supplier: Molekula
Wright's stain for microscopical staining
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ABTS® Peroxidase Substrate Kit, 1-Component Microwell
Supplier: SERACARE LIFE SCIENCES
KPL ABTS 1-component microwell peroxidase substrate develops a blue-green product when reacted with horseradish peroxidase labeled conjugates in microwell plates or tubes. The expected appearance of
the solution is colorless to light green. Acceptable background absorbance of unreacted substrate is ≤0,025, as measured at 405 nm. KPL ABTS 1-component microwell peroxidase substrate is not suitable for membrane or immunohistochemical staining.