"SIGMA ALDRICH MICROSCOPY"

Supplier: SIGMA ALDRICH MICROSCOPY

Benzoyl peroxide (B5907) is specifically formulated as an initiator for these resins.

Supplier: SIGMA ALDRICH MICROSCOPY

2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4,4′-Methylenebis(N,N-dimethylaniline) is an intermediate in dye manufacture and works as a reagent for the determination of lead. It is suitable for the qualitative assay of cyanogens.

Supplier: SIGMA ALDRICH MICROSCOPY

Nigrosin, alcohol soluble. Nigrosin is a black colored powder. It is widely used to manufacture ink and shoe polish. It is also employed for dyeing leather, wood and textiles. In addition, alcohol soluble nigrosin dye is used in carbon papers, typewriter and ribbons. It is used as an ingredient in rubber repairing lacquer.

Supplier: SIGMA ALDRICH MICROSCOPY

Aniline blue is used to stain collagen fibers in tissue sections using the Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. It has also been used to stain and visualize glycosylated proteins.

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Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.

Supplier: SIGMA ALDRICH MICROSCOPY

Tetrazolium blue chloride (BTC) is a colorless tetrazolium compound that upon reduction forms a colored diformazan. The reactivity has been applied to the analysis of reducing compounds including the nanomolar detection of reducing sugars and carbidopa and methyldopa. BTC has been used for histological staining of mouse coronal brain sections and rat brain sections. In combination with 4-nitrocatechol, tetrazolium blue chloride forms a ternary complex with molybdenum(VI) for extraction and spectrophotometric determination of molybdenum. It has also been used to stain Francisella tularensis cells for tularemic microagglutination test.

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Thymol blue sodium salt in ACS Reagent grade water 95% (dye content), Sigma-Aldrich®

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(= 10% Formaline solution)

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3,3′-Dioctadecyloxacarbocyanine perchlorate has been used for lipid bilayer labeling in antigen-presenting cells. It has also been used as a lipophilic dye for labeling in zebrafish embryos.

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Acid Green 25, Dye content ≥60 %

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Carmine is a cochineal product usually furnished as an aluminum and calcium salt of carminic acid. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is suitable for staining nuclei in histological sections. It is utilised in various staining methods including the procedure for detection of glycogen and mucous substances (Best′s carmine), for nuclear staining, or vital staining.

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Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The following are two common stain formulations: 0.1% Ponceau S (w/v) in 5% acetic acid 2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid.

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Calcein blue fluorescent dye, Sigma-Aldrich®

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Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

Supplier: SIGMA ALDRICH MICROSCOPY

Rhodamine B is a xanthene dye, which functions as a water tracer fluorescent. It is used as a colorant in textiles and food stuffs. Rhodamine B is associated with eye, skin and respiratory tract irritation. It is used as a biomarker in oral rabies vaccine.