"SIGMA ALDRICH MICROSCOPY"

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Auramine O, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acridine is a small cationic and planar dye that mainly binds to RNA and DNA. These dyes are strongly fluorescent. It resembles the structure of xanthene except that the heteroatom is nitrogen, not oxygen. Acridine is suitable for nucleic acid staining, particularly for cell cycle determination. Acridines are used as fluorescent materials for visualisation of biomolecules, and in laser technologies.

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Methylene blue, Neisser formulation. Well developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractive bodies within the bacterial cytoplasm. With methylene blue they stain metachromatically, a reddish purple color.

Supplier: SIGMA ALDRICH MICROSCOPY

Building block for 'Anti-Bredt' strained molecules.

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The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

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3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridisation. DAB (3,3′-Diaminobenzidine) is utilised in many applications for the visualisation of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidised DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

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CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

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3,4,5,6-Tetrabromophenolsulfonephthalein, Dye content 95 %

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May-Grünwald stain is a Romanowsky neutraldye stain based on the combination ofmethylene blue azures as the basic dyecomponent and eosin as the acid dyecomponent.It is intended for use in staining blood films orbone marrow films. It is used routinely for diagnostic purposes in hematology.

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Suitable for titration of morpholine

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Kit component in the Masson Trichrome procedure, catalog number HT15

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For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

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5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

Supplier: SIGMA ALDRICH MICROSCOPY

When blood films are stained using Wright-Giemsa Stain, the white blood cell nucleus and cytoplasm take on the characteristic blue or pink coloration. The combination of purified eosin and thiazine dyes in the product eliminates inconsistent staining and yields reproducible chromogenic responses.

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Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates.

Supplier: SIGMA ALDRICH MICROSCOPY

1,9-Dimethyl-Methylene Blue zinc chloride double salt has been used for the quantification of sulfated glycosaminoglycans.