"SIGMA ALDRICH MICROSCOPY"

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescein diacetate (FDA) is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. Fluorescein diacetate has been used for the differentiation of live cells from dead cells.

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Green 25, Dye content ≥60 %

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(= 10% Formaline solution)

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3,3′-Dioctadecyloxacarbocyanine perchlorate has been used for lipid bilayer labeling in antigen-presenting cells. It has also been used as a lipophilic dye for labeling in zebrafish embryos.

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Coproporphyrin I tetramethyl ester. ≥90% (HPLC) (Tetramethyl 3,8,13,18-tetramethyl-21H,23H-porphine-2,7,12,17-tetrapropionate)

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Carmine is a cochineal product usually furnished as an aluminum and calcium salt of carminic acid. Carminic acid is used for human-medical cell diagnosis and serves the purpose of the histological investigation of sample material of human origin. It is suitable for staining nuclei in histological sections. It is utilised in various staining methods including the procedure for detection of glycogen and mucous substances (Best′s carmine), for nuclear staining, or vital staining.

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Sudan orange G belongs to the group of azo dyes, which are synthetic organic colorants. Members of azo dyes contain chromophoric azo groups (-N-N-).

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5,10,15,20-Tetraphenyl-21H, 23H-porphine-p,p′,p,p′′′-tetrasulfonic acid tetrasodium hydrate is a water-soluble porphyrin. It is a negatively charged compound due to the presence of the sulfonate groups. It has been used to functionalize chemically converted graphene to form conductive graphene films of varying thickness.

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Fluorescent label for amino acids allows for detection of alanine, arginine, glycine, glutamic and aspartic acid in the 0.8-4.5 × 10-18 M range.

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Fluorescent probe for hydrophobic (non-polar) regions in proteins and nucleoproteins.

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Formalin solution, neutral buffered, 10% is the most commonly used fixative for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

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Alizarin red S (sodium salt), Sigma-Aldrich®

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Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). For detection of protein bands following electrophoresis.

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Orange II sodium salt,(Certified by the Biological Stain Commission), Dye content ≥85 % (4-(2-Hydroxy-1-naphthylazo)benzenesulfonic acid sodium salt; Acid Orange 7; Acid Orange A; Orange II; Tropaeolin 000 No. 2).

Supplier: SIGMA ALDRICH MICROSCOPY

Orange G (certified), 0.3% and phosphotungstic acid, 0.015%, in denatured alcohol

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoroshield™ with PPD (p-phenylenediamine; 1,4-phenylenediamine) is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.