1077 Results for: "SIGMA ALDRICH MICROSCOPY"

Supplier: SIGMA ALDRICH MICROSCOPY

Used in procedure HT100 for the toning of sections stained with the silver methanamine procedure.

Supplier: SIGMA ALDRICH MICROSCOPY

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4',4"-disulfonic acid sodium salt is also known as ferrozine.

Supplier: SIGMA ALDRICH MICROSCOPY

Dihydrofluorescein diacetate,97% (Diacetyldihydrofluorescein)

Supplier: SIGMA ALDRICH MICROSCOPY

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection. The following are two common stain formulations: 0.1% Ponceau S (w/v) in 5% acetic acid 2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid.

Supplier: SIGMA ALDRICH MICROSCOPY

N,N-Dimethyl-1-naphthylamine is an aromatic amine and a dye.

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Purpurin (1, 2, 4-trihydroxyanthraquinone) is the key pigment present in the roots of Indian madder (Rubia cordifolia). This pigment produces colors, with distinctive heat and light resistant properties.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescent probe for hydrophobic (non-polar) regions in proteins and nucleoproteins.

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3,3′-Dioctadecyloxacarbocyanine perchlorate has been used for lipid bilayer labeling in antigen-presenting cells. It has also been used as a lipophilic dye for labeling in zebrafish embryos.

Supplier: SIGMA ALDRICH MICROSCOPY

2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Gill No. 1 formulation is used as a progressive cytology stain. Used with hematoxylin and eosin staining.

Supplier: SIGMA ALDRICH MICROSCOPY

Gill formulation No. 3 can be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time.

Supplier: SIGMA ALDRICH MICROSCOPY

Clobetasone butyrate is a corticosteroid. It can be used as a diagnostic assay manufacturing, hematology, histology. Clobetasone butyrate has been used as an internal standard for the quantitative assay of clobetasol propionate. Clobetasone butyrate has been used to analyse its bio-concentration factor (BCF) in cyprinus carpio. Clobetasone butyrate is a potent topical corticosteroid and is primarily used to treat atopic dermatitis (AD). Low dosage of this drug is used to treat dry eye in sjogrens syndrome.

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Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). For detection of protein bands following electrophoresis.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromothymol Blue sodium salt has been used as a pH indicator in the yeast growth media (YPD, yeast extract peptone dextrose).

Supplier: SIGMA ALDRICH MICROSCOPY

3-Methoxydiphenylamine can be used in the preparation of 3- methoxydiphenylamine-4-diazonium salts (MDP-4-DS) and the corresponding diazoresins.[1] Diazonium salts and diazoresins find applications in dyes, stains, indicators, general laboratory use, and industrial applications.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescent label for amino acids allows for detection of alanine, arginine, glycine, glutamic and aspartic acid in the 0.8-4.5 × 10-18 M range.

Supplier: SIGMA ALDRICH MICROSCOPY

Lissamine Green B has been used in blood-brain barrier (BBB) studies and is used to study the its effect on herpes simplex virus 1 (HSV-1) corneal infection in animals.

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.

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Orange G (certified), 0.3% and phosphotungstic acid, 0.015%, in denatured alcohol

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Formalin solution, neutral buffered, 10% is the most commonly used fixative for light microscopy. It requires a relatively short period of fixation. It is suitable for the long-term storage of tissue. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship.

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Alginic acid is a high molecular weight linear polymer isolated from seaweed. It is a linear β-1→4-linked polymer of n-mannuronic and L-guluronic acid. It is an acid polysaccharide present in the extracellular matrix of brown algae.

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Fast Blue RR has been used to detect alkaline phosphatase activity.

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Water-soluble, negatively-charged spin trap used to monitor aqueous phase of SDS micelles.

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Readily polymerizes in aqueous solution, forming a stable free radical that inhibits protein-nucleic acid interactions. It is a potent inhibitor of ribonuclease and topoisomerase II by preventing the binding of the nucleic acid to the enzyme. It stimulates tyrosine phosphorylation processes including the Jak2/STAT5 pathway in NB2 lymphoma cells, ErbB4 in neuroblastoma cells, and MAP kinases, Shc proteins, phosphatidylinositide 3-kinase and phospholipase Cγ in PC12 cells. Inhibits apoptosis. It prevents down-regulation of Ca²⁺ -impermeable GluR2 receptors and inhibits calpain, a Ca²⁺ -activated protease that is activated during apoptosis.

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Evans blue has been used to check cell viability. It has been used to study BBB permeability.

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Mordant orange 1 is also known as Alizarin Yellow R, C.I. 14030. Mordant orange 1 is an azo dye. Azo dyes used as biological stains contain an azo group (−N=N−). It is used for colouring many different materials such as textile, leather, and plastics and for manufacturing paints and lacquers and for printings purposes as well.

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4-Phenylazophenol is a polymethacrylates containing polar azo dye. 4-Phenylazophenol (4PP) has been used to study mineralisation by P. chrysosporium under nitrogen-sufficient and nitrogen-limiting culture conditions.

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Mordant Blue 9, Dye content 50 % (6-(5-Chloro-2-hydroxy-3-sulfophenylazo)-5-hydroxy-1-naphthalenesulfonic acid disodium salt; 6-(5-Chloro-2-hydroxy-4-sulfonphenylazo)-5-hydroxy-1-naphthalene-sulfonic acid sodium salt)

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Orange II sodium salt,(Certified by the Biological Stain Commission), Dye content ≥85 % (4-(2-Hydroxy-1-naphthylazo)benzenesulfonic acid sodium salt; Acid Orange 7; Acid Orange A; Orange II; Tropaeolin 000 No. 2).

Supplier: SIGMA ALDRICH MICROSCOPY

Cibacron Brilliant Yellow 3G-P, (Reactive yellow 2)