"Other Essentials"

Supplier: Abcam

Highly sensitive, specific fluorescent detector for nitric oxide (NO). Essentially nonfluorescent until it reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T).

Supplier: Abcam

Amide form of vitamin B₃. Plasma membrane and blood-brain barrier permeable. Precursor for β-nicotinamide adenine dinucleotide (NAD+). Essential for the synthesis of NAD+ phosphate (NADP+). Essential for energy metabolism, normal physiology and disease progression. Associated with a number of signaling pathways including oxidative stress, cell survival and inflammation. These effects are orchestrated partly through forkhead, Akt, Bad, caspase, MAPK and PARP signaling. Non-selective sirtuin inhibitor. Commonly used as 3D growth matrix components and additive for long-term growth.

Supplier: Abcam

Cell permeable reagent for quantification of low concentrations of nitric oxide in solution. Essentially nonfluorescent until it is hydrolyzed to DAF-FM by intracellular esterases and reacts with NO to form a fluorescent benzotriazole.

Supplier: MP Biomedicals

Rich medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium.

Supplier: Abcam

DNA Isolation Kit - Plasma/Serum (ab156893) is a complete set of essential components which enables the experimenter to efficiently isolate DNA from plasma, serum, and body fluids.

Supplier: Abcam

Selective TRPC4 channel blocker (IC₅₀ = 0,96 (fluorescence assay) - 2,6 μM (electrophysiological assay)). Selective versus TRPC6 (19-fold) and TRPC5 (9-fold). Essentially inactive at TRPV1, TRPV3, TRPA1 and TRPM8 channels at concentrations up to 22 μM.

Supplier: Abcam

Essential amino acid which is necessary for normal growth in infants and for nitrogen balance in adults. Used as an antidepressant, anxiolytic and sleep aid. Precursor to niacin, indole alkaloids, serotonin and melatonin.

Supplier: Abcam

Spermine is a biogenic polyamine formed from spermidine and occurs in almost all tissues. It is essential for both neoplastic and normal tissue growth. Spermine is associated with nucleic acids, particularly in viruses, and is thought to stabilize the helical structure. Spermine tetrahydrochloride is used for precipitation of DNA from low salt aqueous buffers.

Supplier: Abcam

Highly sensitive, photo-stable fluorescent probe for quantification of low concentrations of nitric oxide (NO). More sensitive to nitric oxide (NO) than DAF-2, More photo-stable and less pH sensitive than DAF-2, Not as cell-permeable as the diacetate derivative, DAF-FM DA , but it does not need to be activated by cytosolic enzymes. Essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole.

Supplier: Abcam

Widely used blue fluorescent thiol-reactive dye. Essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.

Supplier: Abcam

Essential polyunsaturated fatty acid. PPAR activator. Induces apoptosis. Augments cellular oxidant status and induces mitochondrial dysfunction. Modulates the infla mMatory process and regulates neutrophil function. Stimulates thromboxanes and prostaglandin production. Shows antithrombotic effects in vivo. Orally active.

Supplier: Abcam

Monochlorobimane is a cell-permeant probe for quantifying glutathione levels in cells. It is essentially nonfluorescent until it reacts with a thiol group. It readily reacts with several low molecular weight thiols, including cysteine, glutathione, N-acetylcysteine, mercaptopurine, peptides and plasma thiols. The glutathione conjugate of monochlorobimane has an excitation/emission maxima ~394/490 nM.

Supplier: Abcam

Fluorescent probe for measuring protein conformational changes and quantitative measurement of protein hydrophobicity processes that modify the exposure of the probe to water. Essentially nonfluorescent in water. Fluorescent when bound to membranes (q.y. ~0,27) or hydrophobic domains of proteins (q.y. ~0,72). Fluorescence enhanced by cyclodextrins. Can be used to provide a sensitive method for separating and analyzing cyclodextrins by capillary electrophoresis.

Supplier: Abcam

Essential amino acid for human development. Important in many functions, including acting as a hepatoprotectant, an acetominophen poisoning antidote, and a urinary acidifier. Serves as a chelating agent for heavy metals. Common methyl-group donor in vivo.

Supplier: G-Biosciences

Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Supplier: Abcam

Anti-p63 antibody [4A4] is a mouse monoclonal antibody designed to detect p63, a transcription factor essential for the development and maintenance of epithelial tissues. Validated for immunohistochemistry (IHC) and reacts with human samples. - Cited in over 150 publications - Trusted by researchers since 2002.