Supplier: G-Biosciences

N-(ρ-Maleimidophenyl) isocyanate Heterobifunctional Cross Linkers.

Supplier: G-Biosciences

Sulfodisuccinimidyl tartrate Homobifunctional Cross Linker.

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Disuccinimidyl suberate Homobifunctional Cross Linker.

Supplier: G-Biosciences

Nourseothricin sulphate is a Streptothricin-class antibiotic. It has an extremely broad spectrum of activity against viruses, bacteria, eukaryotic unicellular and complex organisms. Norseothricin contains both Streptothricin D (C₃₁H₅₈N₁₂O₁₀) and Streptothricin F (C₁₉H₃₄N₈O₈) components.

Supplier: G-Biosciences

PBST is commonly used as a wash solution for Western blot membranes and microtiter plate wells in ELISA assays. The PBST is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from membranes and microtiter plate wells without disrupting the antigen/antibody binding reaction.

Supplier: G-Biosciences

AlamarBlue Cell Viability Assay reagent quantitatively measures the proliferation of mammalian cell lines, bacteria and fungi. The dye incorporates an oxidation-reduction (REDOX) indicator that both fluoresces and change colour in response to the chemical reduction of growth medium due to cell growth.

Supplier: G-Biosciences

These C18 spin columns are ready-to-use micro centrifuge columns for peptide clean up and concentration. The columns consist of porous C18 reverse-phase resin that has a particle size of ~15 µm and a pore size of 300 Å. The resin offers highly efficient binding and recovery of peptides and is ideal for mass spectrometry and other peptide related applications.

Supplier: G-Biosciences

The SpinOUT™ GT-100, GT-600 and GT-1200 columns are versatile, spin format columns for the desalting and buffer exchange of protein solutions ranging from 5 µl through to 4 ml sample volumes. The SpinOUT™ columns are available in three MWCO sizes for >700, >6000 or >30 000 dalton proteins and are suitable for samples containing as little as 20 µg protein/ml.

Supplier: G-Biosciences

The amine reactive HOOK™ Agarose Coupling Kit is designed for the simple and efficient coupling of proteins and peptides to a solid support. The kit allows the generation of both a stable and reusable column for the affinity purification of antibodies, proteins and other protein interacting biomolecules.

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Inhibitor of cysteine proteases & mammalian acetylcholinesterase

Supplier: G-Biosciences

Potassium hydroxide 2 mol/l (2 N) in aqueous solution

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GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

Supplier: G-Biosciences

Specifically designed for plant research and supplied with plant specific reagents, including reagents for removal of plant pigments and other natural products that may interfere with protein analysis. Extracts and solubilises nearly all of the proteins from plants, including membrane as well as soluble proteins. Supplied with a strong proprietary chaotropic extraction buffer to solubilise even the most difficult proteins.

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Bromophenol blue

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This Sulphhydryl Immobilisation Kit is designed for the coupling of sulphhydryl containing peptides to a solid agarose support. The sulphhydryl coupling resin columns utilises iodoacetyl groups that specifically react with free sulphhydryls to form covalent, permanent thioether bonds.

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TRIS HCl (tris(hydroxymethyl)aminomethane hydrochloride)

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Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Supplier: G-Biosciences

G-Biosciences offers several different biotechnology-grade agarose powders. Agarose IV is a linear polymer with a very high molecular weight, giving gel structures unlike those of traditional agaroses. This characteristic produces a strong intercatenary interaction, yeilding a gel with very high gel strength and higher exclusion limit.

Supplier: G-Biosciences

The Pearl™ IgG purification kits are designed for the one step purification of IgG antibodies from serum. The supplied Pearl™ IgG purification resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer. The purified IgG molecules can be stored or used in further downstream applications without further clean-up, such as ammonium sulphate precipitation.

Supplier: G-Biosciences

The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

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Prepacked purification columns for protein purification. Supplied in packs of five.

Supplier: G-Biosciences

The Hydrophobic Chromatography kit is designed to teach students the basic principle of hydrophobic chromatography utilising a hydrophobic enzyme. The use of the enzyme allows purification followed by a simple enzyme assay to detect the fractions that contain the enzyme. This lab activity involves preparation of a crude protein extract and running hydrophobic chromatography to isolate the enzyme.

Supplier: G-Biosciences

Dialysis is a routinely used technique in research laboratories to 'change' the solution a biomolecule is dissolved in. Often the buffers used to isolate biomolecules, such as proteins, are not compatible with downstream applications due to high concentrations of reagents, such as salts. A sample is placed in a bag with a semi-permeable membrane and then placed in the new solution or water. Small molecules, such as salt, can pass through the membrane, moving from an area of high concentration to low concentration. The larger molecules, such as proteins, are retained in the bag. Microdialysis is a modification of dialysis to overcome the problem of dialysing small volumes of precious samples. Microdialysis uses small devices designed for small volumes. This kit allows students to study dialysis using patented microdialysis devices.

Supplier: G-Biosciences

Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

Supplier: G-Biosciences

In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyse the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of three functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.

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Saline-Sodium Citrate Hybridization Buffer (20X) controls stringency during the washing steps in Southern blotting, in situ hybridization, DNA microarray or Northern blotting.

Supplier: G-Biosciences

Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.

Supplier: G-Biosciences

Ready-to-Use: Convenient, time-saving formulation ideal for consistent experimental reproducibility.

Supplier: G-Biosciences

HEPES (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulphonic acid)

Supplier: G-Biosciences

Citric acid