28467 Results for: "G-Biosciences"

Supplier: G-Biosciences

N-Decanoyl-N-methyl-D-glucamine (MEGA-10)

Supplier: G-Biosciences

TRITON™ X-100 (Polyethylene glycol tert-octylphenyl ether)

Supplier: G-Biosciences

G-Biosciences' Sodium meta -Periodate is a mild oxidizing agent that converts carbohydrates to activated active aldehydes

Supplier: G-Biosciences

Molecular biology universal kit, contains 25 ml each of ammonium acetate, calcium chloride, EDTA, lithium acetate, magnesium chloride, potassium acetate, potassium chloride, SDS, sodium chloride, TE, Tris pH 7,0 and Tris pH 8,0

Supplier: G-Biosciences

N-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulphonate(sulphobetaine-12, SB-12)

Supplier: G-Biosciences

ASB-16 ≥99%

Supplier: G-Biosciences

Sodium hydroxide 2 mol/l (2 N) in aqueous solution

Supplier: G-Biosciences

A sterile solution of 5M sodium chloride prepared with ACS Grade sodium chloride.

Supplier: G-Biosciences

The FOCUS™ Protein Alkylation Kit is a proteomic grade iodoacetamide reagent kit for blocking thiols by alkylation. The kit is supplied with a proprietary buffer necessary for efficient alkylation of thiols, while minimising reoxidation of the competing thiol pairs in protein samples. Simply add an appropriate amount to reagent solutions for alkylation of protein thiol groups.

Supplier: G-Biosciences

CytoScan™ LDH Cytotoxicity assay quantitatively measures the cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a tetrazolium salt (iodonitrotetrazolium; (INT)) into a red color formazan by diaphorase. The resulting formazan is measured at 490 nm.

Supplier: G-Biosciences

Extracts and solubilises nearly all of the proteins from mammalian samples, including membrane as well as soluble proteins, by a strong chaotropic extraction buffer to solubilise even the most difficult proteins. Suitable for biological samples from animal tissues and adherent and suspension cells.

Supplier: G-Biosciences

The femtoELISA™ kits utilise a non animal protein blocker, NAP-BLOCKER™ that minimises cross-reactivity with researcher's antigens and antibodies. For the detection of HRP, an improved, ultra sensitive, non volatile, stable colorimetric substrate based on tetramethyl benzidine (TMB) is supplied. Reagent does not require hydrogen peroxide that can have detrimental effects on many biological factors, which may ultimately affect the assays.

Supplier: G-Biosciences

The Reversible Copper Stain™ is a single step stain for the rapid detection of proteins resolved on SDS-PAGE.

Supplier: G-Biosciences

Cetrimonium bromide

Supplier: G-Biosciences

Disuccinimidyl tartrate Homobifunctional Cross Linker.

Supplier: G-Biosciences

Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.

Supplier: G-Biosciences

Anti-IgG Rabbit Antibody (HRP (Horseradish Peroxidase))

Supplier: G-Biosciences

Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: G-Biosciences

For the rapid purification of soluble, 6X His or GST tagged proteins.

Supplier: G-Biosciences

Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Supplier: G-Biosciences

RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

Supplier: G-Biosciences

These disposable spin columns are designed for protein purification. These spin columns are uniquely designed, with a snap off end converting to a closure for the column for easy manipulation and use.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: G-Biosciences

LabSafe™ Nucleic Acid Stain is a safe nucleic acid stain for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels.

Supplier: G-Biosciences

This kit teaches aseptic handling techniques and cultivation of bacteria in liquid culture media and on solid phase agar plates. This kit is designed to educate students about the various stages of the bacterial growth cycle, i.e. lag, log or exponential, stationary and decline or death phases. The kit also teaches the importance of growing bacteria on solid phase agar plates to isolate single colonies of bacteria. This lab activity involves preparation of culture medium and solid agar plates.

Supplier: G-Biosciences

In this lab activity, students learn to perform simple protein isolation procedures to isolate the protein fingerprint from various fresh tissues. They will compare the protein fingerprints of four different tissues to understand that the function of a particular organ is due to the proteins that are localised to the specific organ. Also included in this kit are four dried protein samples (from mouse liver, brain, heart and lungs) to compare as a control, if fresh tissues cannot be obtained.

Supplier: G-Biosciences

This lab activity has two objectives - to deepen the understanding of protein molecules and for students to learn the potential of electrophoresis in protein analysis. Students study the fundamentals of protein structure from their primary structure to the more complex tertiary and quaternary structures, utilising protein electrophoresis. Complex mixtures of protein samples and characterised pure protein samples, in conjunction with electrophoresis, are utilised to study protein structure and the potential of protein electrophoresis. Using non-denaturing and denaturing electrophoresis, students understand the difference between primary, tertiary and quaternary structures, the importance of disulfhydryl bridges in maintaining protein structure and electrophoresis in studying complete proteins and protein subunits.

Supplier: G-Biosciences

Ion exchange chromatography is used to separate charged molecules from complex biological samples. The charged molecules bind to a solid support carrying an opposite charge to the molecule. Proteins contain regions of charged groups on the surface which interact with the ion exchange groups immobilised on the solid support (resin column). Immobilised proteins are eluted by changing either pH or the salt gradient or a combination of both. This lab activity involves preparation of a crude protein extract and running ion exchange chromatography for isolation of proteins.