361 Results for: "Electrophoresis Buffers"

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: MP Biomedicals

DL-Dithiothreitol is also known as Clelands reagent; Protective agent for sulfhydryl groups (-SH). Quantitatively reduces disulfides (-S-S- to -SH). In this reaction the DTT is oxidized to the cyclic disulfide which ensures the reduction of other disulfides in solution. Disulfide reduction occurs quickly at pH 8.
Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Useful for stabilizing sulfhydryl-containing enzymes.

Supplier: G-Biosciences

Kit Protein Folding Study includes PAGE mix (19:1 acrylamide/bisacrylamide), ammonium persulfate (APS), detergent solution (10% SDS) , PAGE stacking buffer, PAGE separating buffer, TEMED, sample loading buffer (2X concentrated), electrophoresis runni 1 * 1 KIT

Supplier: Biotium

Go-Go Fast DNA Gel Running Buffer is a high voltage running buffer for agarose gel electrophoresis. With Go-Go Fast, you can run DNA gels up to 300V for 3X faster run times compared to TBE or TAE gels and superior DNA resolution. 200 mL of 50X buffer 1 * 200 mL

Supplier: Biotium

Go-Go Fast DNA Gel Running Buffer is a high voltage running buffer for agarose gel electrophoresis. With Go-Go Fast, you can run DNA gels up to 300V for 3X faster run times compared to TBE or TAE gels and superior DNA resolution.500 mL of 50X buffer 1 * 500 mL

Supplier: Boster Bio

SDS-PAGE Electrophoresis Buffer 1 * 1 L

Supplier: EDVOTEK

electrophoresis buffer 50x tae 1 * 1 items

Supplier: TREVIGEN

20X TTE Electrophoresis Buffer 2 x 500 1 * 1.000 mL

Supplier: EDVOTEK

This powdered blend Tris-Borate EDTA buffer (TBE), has been optimized for agarose gel electrophoresis of nucleic acids. Dissolve pow der in 500 ml of distilled or deionized water to prepare a 10x concentrate which can be diluted 10-fold to make a 1x 1 * 1 items

Supplier: Merck

TRIS ACETATE-EDTA BUFFER SUITABLE FOR ELECTROPHORESIS 1 * 1 L

Supplier: G-Biosciences

Kit Introduction to Agarose Electrophoresis includes DNA plasmid 1, DNA plasmid 1 (digested), DNA plasmid 2, DNA plasmid 2 (digested ), agarose, DNA stain (500X), DNA ladder (1kb), DNA loading buffer (6X), TAE buffer (50X), centrifuge tubes (2ml). 1 * 1 KIT

Supplier: G-Biosciences

molecular grade, concentrated running buffer for dna and rna electrophoresis. 1 * 250 mL

Supplier: Merck

TRIS ACETATE-EDTA BUFFER DNASE AND RNASE NONE DETECTED, BIOREAGENT, SUITABLE FOR ELECTROPHORESIS, 10× CONCENTRATE 1 * 1 L

Supplier: G-Biosciences

lds sample loading buffer [4x] is used to prepare protein samples for sds polyacrylamide gel electrophoresis. 1 * 10 mL

Supplier: EMBITEC

MiniLab a taste of genetics - Extraction, PCR and electrophoresis reagents. Includes DNA extraction solution, Taq polymerase, primers, restriction enzyme, GreenGel cups, running buffer and accessoires for ten groups of students. 1 * 1 KIT

Supplier: EMBITEC

MiniLab NGSS aligned colour dyes & gel electrophoresis. Includes ten 1% agarose gel cups, six color dye samples, one set of filter p apers and green food coloring for exploring paper chromatography, one bottle of 100 mL Tris-Borate-EDTA (TBE) buffer 1 * 1 KIT

Supplier: HOEFER ELECTROPHORESIS

Vertical gel electrophoresis system

Supplier: HOEFER ELECTROPHORESIS

Vertical gel electrophoresis system

Supplier: Corning

Axygen® horizontal gel box is a high quality, full-featured unit designed for exceptional durability and efficiency. These gel boxes feature leak-free external gel casting, which allows the user to cast one gel outside of the box while another gel is running inside. Boxes from 7, 10, 15 and 20cm

Supplier: THERMO OWL SCIENTIFIC

The Owl™ D2 wide gel system is ideal for screening PCR samples or for routine DNA fragment analysis.

Supplier: THERMO OWL SCIENTIFIC

The durable, easy‑to‑use, Owl™ B1 EasyCast™ mini horizontal gel system is suitable for running between five and 28 samples and is ideal for most teaching applications.

Supplier: PanReac AppliChem

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.3% suitable for buffer solutions

Supplier: EKSIGENT

he dsDNA 1000 Kit is designed for use on the PA 800 Plus and contains the supplies necessary to perform a high-performance separation and analysis of double-stranded DNA (dsDNA) fragments.

Supplier: PanReac AppliChem

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9%, Ultrapure

Supplier: PanReac AppliChem

Tris(hydroxymethyl)aminomethane (TRIS, Trometamol) ≥99.9% for molecular biology

Supplier: Cytiva (Formerly Pall Lab)

These centrifugal devices provide a precise, quick recovery of microlitre volumes of concentrate.

Supplier: VWR Chemicals

TEMPase Hot Start DNA Polymerase Master Mix and Blue TEMPase Master Mix are good alternatives to TEMPase Hot Start DNA Polymerase. These master mixes offer easy reaction assembly at room temperature, reduced set-up time and fewer handling steps, which lead to increased reproducibility. As a consequence TEMPase Hot Start DNA Polymerase Master Mix is highly suited to standard tests.

Supplier: Quantabio

The AccuStart™ II Mouse Genotyping kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with AccuStart™ II GelTrack™ PCR SuperMix in a single kit.

Supplier: Quantabio

AccuStart™ II PCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It contains all components, except primers and template.

Supplier: MP Biomedicals

The Fast DNA® SPIN Kit for Faeces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.