361 Results for: "Electrophoresis Buffers"

Supplier: Thermo Fisher Scientific

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

Supplier: EMBITEC

The ultra compact RunOne™ electrophoresis system is able to run a whole variety of gel sizes from mini to long in less than 350 ml of buffer. The power supply with selectable 25, 50 or 100 V output slides directly into the running unit. The system can run eight to 108 samples in minutes, with the separate casting system allowing up to six gels to be cast and run at the same time.

Supplier: Cytiva

Tris (tris[hydroxymethyl] aminoethane) is suitable for preparing electrophoresis buffer in the pH range of 7,2 to 9,0. It is qualified for 2-D electrophoresis.

Supplier: Thermo Fisher Scientific

Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.

Supplier: G-Biosciences

The femto TBST™ and femto PBST™ wash buffers will enhance sensitivity of immunoassays by minimising the 'washing out' of immuno agents or immuno complexes, a common problem associated with classical TBST and PBST buffers. The wash buffers aid in the generation of cleaner backgrounds, resulting in a larger ratio of signal to background. These can be used for incubation and washing procedures.

Supplier: MP Biomedicals

Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.

Supplier: Agilent

CEP coated capillaries contain a permanently bonded polymer coating. This CEP coating shields the silanol functionality of the capillary surface and helps prevent sample adsorption. Additionally, EOF is nearly eliminated, making the capillary ideal for applications such as DNA separations with sieving polymer buffers.

Supplier: MP Biomedicals

Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.

Supplier: Agilent

CE/MS sprayer kit and CE/MS adapter kit to couple an Agilent CE instrument with an MS system.

Supplier: THERMO OWL SCIENTIFIC

The Owl™ B2 EasyCast™ mini horizontal gel system provides flexibility in a small footprint and is available with or without buffer exchange ports. The Owl™ B3 with built-in recirculation system prevents formation of pH and ionic gradients for high resolution and uniform reproducible results. The Owl™ B3 is ideal for long runs, multiple sample sets or RNA gels. It also eliminates uneven migration, band distortion or disassociation of pH dependent glycoxylated RNA molecules that can result when ionic depletion occurs. As the recirculation system is built into the buffer chamber, no external pumps, tubing or stirring bars are required.

Supplier: EKSIGENT

SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.

Supplier: MP Biomedicals

Sodium acetate is used as a buffer in the buffering range of pH 3.6-5.6. It is also used in the purification and precipitation of nucleic acids, protein crystallization, staining of gels in protein gel electrophoresis, HPLC, mordant dyeing, DNA microarray studies of E. coli response, investigation of protein unfolding during reverse phase chromatography.

Supplier: G-Biosciences

Simply heat and use for sealing the IPG strips for SDS-PAGE analysis. The Agarose Sealing Solution is prepared in a proprietary buffer to minimise reoxidation of the competing thiol pairs as proteins enter into the second dimension gel. Improves resolution and prevents streaking of protein spots on 2D gels.

Supplier: Thermo Fisher Scientific

Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Supplier: Cytiva

DIGE gel is a 12,5% precast polyacrylamide gel in a low fluorescent glass cassette for second dimension 2-D electrophoresis and specially developed for 2-D DIGE analysis. DIGE gel and DIGE buffer kit are tools to increase the quality, reproducibility, and convenience of 2-D DIGE using Ettan™ DIGE system.

Supplier: G-Biosciences

Using the Protein Electrophoresis kit, students will learn the principles of various types of electrophoresis, including denaturing and non-denaturing electrophoresis, and how this powerful technique is used to analyse proteins. The kit will introduce students to the different separation matrices currently in use and will understand their differing separation properties and their role in protein analysis. Students have an option of casting their own electrophoresis gels using polyacrylamide or using pre-cast commercially available gels. This kit is provided with all of the reagents, buffers and supplies needed for casting acrylamide gels, preparing protein samples, running electrophoresis, and staining the gels for visualisation of protein bands. Test protein samples and protein standards are also provided.

Supplier: THERMO OWL SCIENTIFIC

The Owl™ B1A EasyCast™ mini gel system is designed to provide flat, even banding patterns and consistent results with minimum effort. This system is ideal for determination of molecular weight ranges of PCR fragments or larger sized DNA molecules. The all-in-one design allows the option of casting and running a gel in the buffer chamber, eliminating the need for additional equipment.

Supplier: Cytiva

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
Glycine is a non-essential amino acid. It is only amino acid with no asymmetric carbon and thus is not chiral. It is the major inhibitory neurotransmitter. It is involved in the biosynthesis of the porphyrin rings of hemes and chlorophylls.
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15 - 30 °C)
Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component. Glycerol is used in the concentration and storage of enzymes. Also prevents back-diffusion and protein samples into the buffer. Ideal for use as a cryoprotectant.

Supplier: MP Biomedicals

Tris and Tris Hydrochloride have been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.

Supplier: Agilent

PVA coated capillaries contain a permanently adsorbed layer of polyvinyl alcohol. This coating minimises hydrophobic and electrostatic solute/wall interactions and eliminates electroosmotic flow (EOF). Using a proprietary deposition process, the PVA coating is stable over a wide pHrange, even under basic conditions from 2,5 to 9,5. This stability allows the use of many common CE buffers. Because the silica surface is covered, many proteins and amines can be analysed without the peak tailing found with uncoated capillaries. In addition, since EOF is eliminated, cumbersome washing procedures are unnecessary and migration time reproducibility may be improved.

Supplier: MP Biomedicals

Tris has been useful as buffers in a wide variety of biological systems. Uses include pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications. Tris has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). Tris does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is an auxiliary material in pharmaceutical science.
Store at Room Temperature (15-30 °C). Store dessicated.

Supplier: MP Biomedicals

Borax is a salt of boric acid which is used as a buffer. It has a co-complexing ability with other agents in water to form complex ions with various substances. It is also commonly utilized in chromatography and electrophoresis studies.

Supplier: LONZA

FlashGel™ system for RNA is optimised for the unique requirements of RNA, and is an ideal tool for rapid analysis of sample integrity. High quality, intact RNA is essential for consistent results in gene expression, Northern analysis, cDNA library construction and cDNA labelling for microarrays. Most protocols recommend checking RNA prior to downstream analysis.

Supplier: MP Biomedicals

Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis.

Supplier: MP Biomedicals

Tris have been useful as buffers in a wide variety of biological systems. It has been used as a starting material for polymers, oxazolones (with carboxylic acids) and oxazolidines (with aldehydes). It does not precipitate calcium salts and is of value in maintaining solubility of manganese salts. It can be used for the direct standardization of a strong acid solution; the equivalence point can be determined either potentiometrically or by use of a suitable indicator such as 3-(4-Dimethylamino-1-naphthylazo)-4-methoxybenzenesulfonic acid. It is RNAse and DNAse-free. Tris is relatively non-hygroscopic ; but, if needed, it can be dried at 100 °C for up to 4 hours to remove any water.
Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions.

Supplier: MP Biomedicals

This product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rabbit IgG (whole molecule) and buffer salts. Anti-Human IgG is developed in goat using IgG from pooled normal human serum as the immunogen. Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other goat serum proteins. Specificity for human IgG is determined by immunoelectrophoresis (IEP) versus purified human IgA, IgG, IgM, Bence Jones kappa, and Bence Jones lambda myeloma proteins.Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to conjugation. Electrophoresis of the antibody preparation followed by diffusion versus anti-goat IgG and anti-goat whole serum result in single arcs of precipitation in the gamma region.

Supplier: THERMO OWL SCIENTIFIC

The Owl™ A2 horizontal gel system is a simple, convenient and fast system for detailed DNA/RNA analysis on multiple samples. It is available with or without buffer exchange ports. The Owl A5 large gel system with built-in recirculation prevents formation of pH and ionic gradients for high resolution and uniform reproducible results. The Owl A5 is ideal for long runs, multiple sample sets or RNA gels. It also eliminates uneven migration, band distortion or disassociation of pH dependent glycoxylated RNA molecules that can result when ionic depletion occurs.