16885 Results for: "ENZO LIFE SCIENCES"

Supplier: Enzo Life Sciences

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Supplier: Enzo Life Sciences

Synapsin I, II and III belong to a family of neuron-specific phosphoproteins which maintain a reserve pool of vesicles in the vicinity of the active zone by tethering synaptic vesicles to each other and to an Actin-based cytoskeleton meshwork. They have been shown to regulate neurotransmitter release in mature synapses and to accelerate development of the nervous system.

Supplier: Enzo Life Sciences

Syntaxins are type II integral membrane proteins localized to membranes that regulate vesicle transport, protein maturation, and exocytosis. Syntaxin 1A resides in the nerve terminals of sensory neurons and nerve fibers reaching small blood vessels. Syntaxin 2 localizes to the apical plasma membrane and regulates epithelial-mesenchymal interactions. Syntaxin 6 is primarily localized to the trans-Golgi network, and partially co-localizes with AP-1 on clathrin-coated membranes. Syntaxin 13 is ubiquitously expressed and localizes to the tubular early and recycling endosomes, as well as endosomal vacuoles.

Supplier: Enzo Life Sciences

Transferrin is a serum glycoprotein involved in iron transport. Transferrin exists in two forms, the iron-free Apotransferrin, which binds two Fe3+ ions to generate Ferrotransferrin. Apotransferrin remains tightly bound to its receptor until the complex is recycled to the cell surface, where Apotransferrin is released into the extracellular space to recruit more Fe3+ ions.

Supplier: Enzo Life Sciences

Anti-HSP90B1 Rat Monoclonal Antibody [clone: 9G10]

Supplier: Enzo Life Sciences

The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The core histones form an octamer, which is made up of one H3-H4 tetramer and two H2A-H2B dimers. The N-terminal tail of core histones undergoes different post-transcriptional modifications including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.

Supplier: Enzo Life Sciences

Disrupts Wnt signaling

Supplier: Enzo Life Sciences

Catenin blocker

Supplier: Enzo Life Sciences

SignaSure® Wash Buffer is formulated for use with alkaline phosphatase-linked detection reagent assay systems.

Supplier: Enzo Life Sciences

Host: Mouse, Isotype: IgG1

Supplier: Enzo Life Sciences

PATHO-GENE® HPV type 16/18/31/33/51 probe is a mixture of biotin-labeled HPV-16, HPV- 18, HPV-31, HPV-33 and HPV-51 specific probes in buffered formamide and hybridisation enhancers.

Supplier: Enzo Life Sciences

In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.

Supplier: Enzo Life Sciences

The PATHO-GENE® Human papillomavirus (HPV) in situ screening assay for tissue sections provides reagents and materials for (a) preparation and pretreatment and (b) hybridization/ detection and typing of HPV DNA (using alkaline phosphatase-NBT/BCIP for signal generation) in 20 tissue biopsy specimens. This product is for research use only and is not to be used for diagnostic procedures.

Supplier: Enzo Life Sciences

Amine reactive dye

Supplier: Enzo Life Sciences

Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .

Supplier: Enzo Life Sciences

Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.

Supplier: Enzo Life Sciences

Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids

Supplier: Enzo Life Sciences

Produced in E. coli.

Supplier: Enzo Life Sciences

Produced in E. coli.

Supplier: Enzo Life Sciences

Digoxigenin NHS ester is a non-radioactive immune-tag, widely used for in situ hybridisation and protein labeling.

Supplier: Enzo Life Sciences

MITO-ID® Red Detection Kit (GFP-CERTIFIED®) is a photostable, non-toxic and selective mitochondrial dye that stains live, detergent permeabilised and aldehyde fixed cells. This kit is specifically designed for use with GFP expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFP's, CFP's, YFP's).

Supplier: Enzo Life Sciences

Calcium dye

Supplier: Enzo Life Sciences

MITO-ID® Green Detection Kit is a potent mitochondria-selective dye suitable for live, permeabilised and fixed cell staining.

Supplier: Enzo Life Sciences

CpG oligodeoxynucleotide (Type B) with phosphorothioate backbone. Specific ligand for human and mouse TLR9 (Toll-like receptor 9).

Supplier: Enzo Life Sciences

PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.

Supplier: Enzo Life Sciences

Highly quenched and sensitive fluorogenic MMP substrate

Supplier: Enzo Life Sciences

NLRP3 inflammasome activator.

Supplier: Enzo Life Sciences

The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATP-dependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, alpha and beta, each group having distinct structural and functional roles. The alpha-subunits comprise the outer rings and the beta-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (alpha7-beta7-beta7-alpha7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the alpha- or beta-rings .
In addition to the 20S particle, the 26S complex contains over twenty additional proteins, ranging in molecular weight from 25 to 10kDa, located in a distinct complex called the ‘PA700 proteasome activator’ or the ‘19S complex’, and which determines substrate specificity and provides the multiple enzymatic functions necessary for proteolysis and viability. Systematic analysis of the sub-unit components have revealed at least six members to be ATPases belonging to a new family of ATPbinding proteins, together with a further fifteen sub-units that lack the capacity to bind ATP, isopeptidases and several other proteins thought to be responsible for the unfolding of a protein substrate prior to insertion into the proteolytic core of the 20S proteasome.

Supplier: Enzo Life Sciences

Host: Rabbit

Supplier: Enzo Life Sciences

The proteasome is widely recognised as the central enzyme of non-lysosomal protein degradation. It is responsible for intracellular protein turnover and it is also critically involved in many regulatory processes and, in higher eukaryotes, in antigen processing. The 26S proteasome is the key enzyme of the ubiquitin/ATPdependent pathway of protein degradation. The catalytic core of this unusually large (2000kDa, 450Å in length) complex is formed by the 20S proteasome, a barrel shaped structure shown by electron microscopy to comprise of four rings each containing seven subunits. Based on sequence similarity, all fourteen 20S proteasomal subunit sequences may be classified into two groups, α and β, each group having distinct structural and functional roles. The α-subunits comprise the outer rings and the β-subunits the inner rings of the 20S proteasome. Observations of the eukaryotic proteasome and analysis of subunit sequences indicate that each ring contains seven different subunits (α7β7β7α7) with a member of each sub-family represented in each particle. Each subunit is located in a unique position within the α- or β-rings. In addition to the 20S particle, the 26S complex contains over twenty additional proteins, ranging in molecular weight from 25 to 10kDa, located in a distinct complex called the ‘PA700 proteasome activator’ or the ‘19S complex’, and which determines substrate specificity and provides the multiple enzymatic functions necessary for proteolysis and viability. Systematic analysis of the sub-unit components have revealed at least six members to be ATPases belonging to a new family of ATP-binding proteins, together with a further fifteen subunits that lack the capacity to bind ATP, isopeptidases and several other proteins thought to be responsible for the unfolding of a protein substrate prior to insertion into the proteolytic core of the 20S proteasome.