"DL-Cystine&pageNo=26&view=easy"

Supplier: Thermo Fisher Scientific

Excellent colorimetric substrate for detection of horseradish peroxidase labelled probes and is used with peroxidase and peroxidase coupled systems, particularly in ELISA techniques, blue result.

Supplier: USP

USP Reference Standards are specified for use in conducting official USP–NF tests and assays. To confirm accuracy and reproducibility, USP Reference Standards are rigorously tested and evaluated by multiple independent laboratories including USP, commercial, regulatory, and academic labs. USP also provide publicly available, official documentary standards for pharmaceutical ingredients in the USP–NF that link directly with our primary reference standards.

Supplier: Thermo Scientific

2,4-Dichloro-6-methylbenzenesulphonyl chloride

Supplier: Merck

N-Benzylmaleimide, Sigma-Aldrich®

Supplier: VWR Collection

TEMPERATURE PROBE FOR VWB 2 - 26 1 * 1 items

Supplier: Edmund Bühler GmbH

Bench-top orbital shaker with heating plate which is suitable for mixing of difficult media. This shaker is the ideal solution when the use of an incubator hood is not possible due to the use of solvents (as solvents could destroy electrical parts and other materials of the incubator hood). The unit features digital display of set and actual values and programmable control.

Supplier: Bioss

c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

Supplier: EHRENSTORFER

Bisphenol F Bis(2,3-dihydroxypropyl) ether

Supplier: Bioss

Matrix Metalloproteinase 28, also known as epilysin, was first cloned from human skin keratinocytes and described to reflect its role in the remodeling of the epidermis. It was found in testis, as well as heart, brain, placenta, lung, prostate, intestine, and colon. MMP28 was later cloned from human lung and found in lung, kidney, brain, skeletal muscle, and several tumor cell lines. At least three MMP28 transcripts of 2.6, 2.0, and 1.2 kb have been reported possibly representing alternatively spliced forms of MMP28. There are two human sequences (isoform1 and isoform 2) which encode proteins of 520 and 393 amino acids with predicted masses of 58.9 and 44.5 kDa respectively. The mouse sequence contains two inserts of 41 and 39 amino acids respectively, relative to the human sequence. Mouse epilysin has a predicted molecular mass of approx. 70 kDa. Mouse and human epilysin are highly conserved and share 97% identical residues. Epilysin (MMP-28) contains the key domains of the other MMPs: a signal peptide, conserved cysteine-containing prodomain (with a furin cleavage site), conserved histidine-containing catalytic domain, hinge domain, and hemopexin domain. MMP28 has a furin cleavage site, similar to MMP11, and is cleaved by the prohormone convertase family of enzymes. Sequence identity between MMP28 and the other MMPs is low overall. MMP28 is most closely related to MMP19 (39% sequence identity). Recombinant MMP28 degrades casein.

Supplier: Bioss

c-Src tyrosine kinase plays a critical role in signal transduction downstream of growth factor receptors, integrins and G protein-coupled receptors. We used stable isotope labeling with amino acids in cell culture (SILAC) approach to identify additional substrates of c-Src tyrosine kinase in human embryonic kidney 293T cells. We have identified 10 known substrates and interactors of c-Src and Src family kinases along with 26 novel substrates. We have experimentally validated 4 of the novel proteins (NICE-4, RNA binding motif 10, FUSE-binding protein 1 and TRK-fused gene) as direct substrates of c-Src using in vitro kinase assays and cotransfection experiments. Significantly, using a c-Src specific inhibitor, we were also able to implicate 3 novel substrates (RNA binding motif 10, EWS1 and Bcl-2 associated transcription factor) in PDGF signaling. Finally, to identify the exact tyrosine residues that are phosphorylated by c-Src on the novel c-Src substrates, we designed custom peptide microarrays containing all possible tyrosine-containing peptides (312 unique peptides) and their mutant counterparts containing a Tyr -->Phe substitution from 14 of the identified substrates. Using this platform, we identified 34 peptides that are phosphorylated by c-Src. We have demonstrated that SILAC-based quantitative proteomics approach is suitable for identification of substrates of nonreceptor tyrosine kinases and can be coupled with peptide microarrays for high-throughput identification of substrate phosphopeptides.

Supplier: Abcam

Antioxidant agent (IC₅₀ = 2 μM, copper-mediated oxidation of LDL). Antiproliferative agent. Activates caspase-3 and caspase-9. Modulates PARP-1 protein. Induces cytochrome c release. Shows apoptotic, antibacterial, antifungal, anticancer, phytoestrogenic, neuroprotective, antihyperglycemic and anti-infla mMatory effects in vivo. Orally active.

Supplier: EDQM

Organic Standard, Imatinib impurity A, (2E)-3-(dimethylamino)-1-(pyridin-3-yl)prop-2-ene-1-one, Ph. Eur. standard

Supplier: ISOPAD

Isopad IT-TeMS is a preterminated and PTFE insulated flexible heating tape.

Supplier: Abcam

Suitable as a fluorogenic substrate for phosphatases. Fluorescence: λex 319 nm; λem 384 nm (pH 9,0); λex 360 nm; λem 449 nm (Reaction product).

Supplier: EHRENSTORFER

Eprinomectin

Supplier: Merck

Toluene-[D₈], Sigma-Aldrich®