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Protein blocking agent, BLOT-QuickBlocker™

Protein blocking agent, BLOT-QuickBlocker™

Supplier: G-Biosciences

BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.

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Millipore® Immobilon®-P NOW Bundle Hydrophobic PVDF Transfer Membrane

Millipore® Immobilon®-P NOW Bundle Hydrophobic PVDF Transfer Membrane

Supplier: Merck

Hydrophobic immobilon-P PVDF transfer membrane for western blotting using chemiluminescence, chromogenic or radioactive detection methods.

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Chemiluminescent detection reagents, Immobilon® Western HRP substrates

Chemiluminescent detection reagents, Immobilon® Western HRP substrates

Supplier: Merck

Immobilon® Western HRP substrates are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.

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VWR® Phosphate Buffered Saline (PBS), High Phosphate

VWR® Phosphate Buffered Saline (PBS), High Phosphate

Supplier: VWR Chemicals

Irradiated solution, pH 7,2, for washing blots.

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Upstate® Autorad Orientation Markers for Western Blotting

Upstate® Autorad Orientation Markers for Western Blotting

Supplier: Merck

Autorad orientation markers for use on immunoblots in detection of Chemiluminescence in Western blotting, dot/slot blotting.

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picoLUCENT™ PLUS-AP kit

picoLUCENT™ PLUS-AP kit

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

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BLOT-FastStain™

BLOT-FastStain™

Supplier: G-Biosciences

BLOT-FastStain™ is a unique stain for reversible staining of protein on nitrocellulose and PVDF transfer membranes. It only stains protein and leaves the background absolutely untouched and brilliant white resulting in high band visibility.

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SNAP i.d.® 2.0 Protein Detection Systems

SNAP i.d.® 2.0 Protein Detection Systems

Supplier: Merck

Developed to meet the western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology and a built-in flow distributor active.

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Blocking buffers

Blocking buffers

Supplier: Rockland Immunochemicals

A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.

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VWR®RapidBlock™, Blocking solution, 10x

VWR®RapidBlock™, Blocking solution, 10x

Supplier: VWR Chemicals

RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.

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Millipore® Immobilon® NOW Dispenser for Transfer Membrane

Millipore® Immobilon® NOW Dispenser for Transfer Membrane

Supplier: Merck

The Immobilon NOW dispenser is ideal for safe storage and convenient use of 8,5 cm wide rolls of transfer membrane for Western blotting. With this device, membrane can be efficiently cut to standard sizes for mini- and midi-blots with no waste.

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Caspase-3 substrates, NucView®

Caspase-3 substrates, NucView®

Supplier: Biotium

NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

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Protein ladders (pre-stained), ScanLater™

Protein ladders (pre-stained), ScanLater™

Supplier: Molecular Devices

The ScanLater™ Western blot protein ladder is a mixture of seven biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD.

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Millipore® Immobilon®-PSQ NOW Bundle Hydrophobic PVDF Transfer Membrane

Millipore® Immobilon®-PSQ NOW Bundle Hydrophobic PVDF Transfer Membrane

Supplier: Merck

PVDF transfer membrane hydrophobic for western blotting using chemiluminescence, chromogenic or radioactive detection methods.

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Phosphate buffered saline (PBS), 10X

Phosphate buffered saline (PBS), 10X

Supplier: G-Biosciences

PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

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SNAP i.d. 2.0 Protein Detection Systems Mini and Midi

SNAP i.d. 2.0 Protein Detection Systems Mini and Midi

Supplier: Merck

Developed to meet the needs of our Western blotting customers, the SNAP i.d. 2.0 system produces blots of a very high quality. Unique vacuum-driven technology and a built-in flow distributor actively drive reagents through the membrane.

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VWR®, Replacement Cables Electrophoresis Systems

Supplier: VWR Collection

Replacement cables suitable for all PerfectBlue™ electrophoresis systems and blotting systems, and are compatible with the majority of commercially available power supplies.

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Nitrotyrosine BSA for immunoblotting experiments

Nitrotyrosine BSA for immunoblotting experiments

Supplier: Cayman Chemical

This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.

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Millipore® Immobilon®-NC Nitrocellulose Transfer Membranes

Supplier: Merck

Immobilon-NC (HATF) membrane is composed of a mixed cellulose esters matrix. It is devoid of surfactants that may interfere with cell wall integrity during cell growth

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Anti-IgG (H+L) Donkey Polyclonal Antibody

Anti-IgG (H+L) Donkey Polyclonal Antibody

Supplier: Rockland Immunochemicals

Anti-Sheep IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

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Millipore® Immobilon®-E NOW Bundle Hydrophobic PVDF Transfer Membrane

Millipore® Immobilon®-E NOW Bundle Hydrophobic PVDF Transfer Membrane

Supplier: Merck

The first PVDF transfer membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

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Anti-MAD2L2 Rabbit Polyclonal Antibody

Anti-MAD2L2 Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemicals

This affinity purified antibody has been tested for use in ELISA, immunochemistry and western blot.  For western blotting HeLa whole cell lysates and nuclear extracts are suggested.  Reactivity in other immunoassays is unknown.

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Anti-IgG (H+L) Goat Polyclonal Antibody

Anti-IgG (H+L) Goat Polyclonal Antibody

Supplier: Rockland Immunochemicals

Anti-Rabbit IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user

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Anti-IgG (H+L) Goat Polyclonal Antibody

Anti-IgG (H+L) Goat Polyclonal Antibody

Supplier: Rockland Immunochemicals

Anti-Mouse IgG whole molecule is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

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Anti-IgM µ Goat Polyclonal Antibody

Anti-IgM µ Goat Polyclonal Antibody

Supplier: Rockland Immunochemicals

Anti-Human IgM mu heavy chain is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

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Anti-GLI1 Rabbit Polyclonal Antibody

Anti-GLI1 Rabbit Polyclonal Antibody

Supplier: Rockland Immunochemicals

This antibody has been tested for use in ELISA, immunochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kDa in size corresponding to Gli-1 Protein by western blotting.

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Gel documentation system, G:BOX Mini

Gel documentation system, G:BOX Mini

Supplier: Syngene (a division of the Synoptics group)

The G:BOX mini is a compact, multi application imaging system for accurately imaging mini and midi fluorescence and visible gels, multiplex fluorescence Western blots, stain free gels and chemiluminescent blots.

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Peqlab peqGOLD, Agarose, Universal

Peqlab peqGOLD, Agarose, Universal

Supplier: VWR Chemicals

High quality standard agarose for routine analysis with broad separation range (0,05 to 50 kbp). Ideal for blotting applications due to high gel strength. Free from RNases, DNases and enzyme inhibitors.

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CHO Host Cell Protein Standards

Supplier: ENZO LIFE SCIENCES

Quantitative protein standards for Western blotting internal controls or ELISA standards.

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VWR® UltraPure, PBS and TBS wash buffers

VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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