"Biowest"

Supplier: Biowest

Penicillin-Streptomycin acts against Gram-negative and Gram-positive bacteria.

Supplier: Biowest

Lymphosep is a standardised, high quality medium designed for simple and rapid isolation of PBMCs from treated whole blood. Lymphosep, like Ficoll™, has the optimised density 1,077 g/ml for lymphocyte separation to guarantee best and reliable results for your preparations.

Supplier: Biowest

These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.

Supplier: Biowest

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: Biowest

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

Supplier: Biowest

Gentamycin sulphate acts against Gram-negative, Gram-positive bacteria and mycoplasma.

Supplier: Biowest

Gentamicin sulfate acts against gram-negative, gram-positive bacteria and mycoplasma.

Supplier: Biowest

Ham's F10 medium is intended to support the growth of Chinese Hamster Ovary cells under serum-free conditions and other mammalian cell types with serum supplementation. It is a popular medium for growth of fastidious cell lines.

Supplier: Biowest

Cell culture water is high-quality water suitable for use as a solvent in the preparation of cell culture media and laboratory reagents.

Supplier: Biowest

Penicillin-Streptomycin acts against gram-negative, gram-positive bacteria and mycoplasma.

Supplier: Biowest

McCoy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy's 5A media supports the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

Supplier: Biowest

Antibiotic-antimycotic used in tissue culture to prevent growth of bacteria (gram positive and gram negative), yeast and fungi. Penicillin acts by inhibiting bacterial cell wall synthesis.

Supplier: Biowest

FreeAdd 1X is a fully chemically defined substitute for animal serum. It provides the necessary nutritional support for cell growth, development and expression. It has been tested on a variety of cells and can be used in combination with basal medium to generate equal or even better cell culture performance.

Supplier: Biowest

Newborn Calf Serum (NBCS) originates from 3 to 10 day old calves. It is a high quality, cost effective replacement for Foetal Bovine Serum (FBS) and contains a somewhat higher proportion of proteins and immunoglobulin, but fewer growth factors. With proper cell adaptation, it has been shown to perform very well in cell culture applications where routine cell growth and cell culture maintenance is the objective.

Supplier: Biowest

Donkey serum may be used as a blocking agent and controls in immunoassays. For immunohistochemistry and immunocytochemistry, donkey serum was used between 5 to 20% as a blocking agent in various studies.