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"Biotium"

 
 

NBD C6-Sphingomyelin membrane stain, for histology

Supplier: Biotium

NBD C6-sphingomyelin (6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)sphingosyl phosphocholine) has been used for following sphingolipid metabolism in cells.

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Biotin-AM3-25 membrane stain, for histology

Supplier: Biotium

Biotin-AM3-25 is a conjugate of AM3-25 and biotin. Membranes labeled with the probe can subsequently couple to avidin or streptavidin.

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Octadecyl rhodamine B chloride (R 18) membrane stain, for histology

Supplier: Biotium

The fluorescence of octadecyl rhodamine B in membranes is quenched at high dye concentration but is released at dilution. This property makes the dye useful for membrane fusion assays.

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Di-2-ANEPEQ (JPW 1114) membrane stain, for histology

Supplier: Biotium

Di-2-ANEPEQ, also called JPW1114, is a fast-responding membrane potential dye. It is highly water-soluble and is usually introduced into cells by microinjection.

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Di-8-ANEPPQ membrane stain, for histology

Supplier: Biotium

Di-8-ANEPPQ is a fast-responding membrane potential dye. It is more hydrophobic than Di-2-ANEPEQ and is useful for potential-sensitive retrograde labeling of neurons.

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Di-12-ANEPPQ membrane stain, for histology

Supplier: Biotium

Di-12-ANEPPQ is a fast-responding membrane potential dye. It is more hydrophobic than Di-8-ANEPPQ and is useful for potential-sensitive retrograde labeling of neurons.

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RH 414 membrane stain, for histology

Supplier: Biotium

The styryl dye RH414 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons.

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RH 237 [N-(4-Sulphobutyl)-4-(6-(4-(dibutylamino)phenyl)hexatrienyl)pyridinium inner salt] membrane stain, for histology

Supplier: Biotium

The styryl dye RH237 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. Excitation/emission data (below) is for the dye in methanol. In cell membranes, the spectra of styryl dyes are typically blue-shifted by as much as 20 nm for absorption or excitation and 80 nm for emission.

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RH795 membrane stain, for histology

Supplier: Biotium

The styryl dye RH795 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. The dye is spectrally similar to RH414 (#61016), but exhibits different physiological effects during staining. For example, RH414 causes arterial constriction during cortex staining, while RH795 does not. The excitation/emission data below are for the dye in methanol. In cell membranes, the spectra of styryl dyes are typically blue-shifted by as much as 20 nm for absorption or excitation and 80 nm for emission.

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DiOC₅(3) iodide membrane stain, for histology

Supplier: Biotium

DiOC5(3) and DiOC6(3) are two of the most widely used carbocyanine dyes used for membrane potential measurements.

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3,3'-Diethyloxacarbocyanine iodide [DiOC2(3) iodide] membrane stain, for histology

Supplier: Biotium

DiOC2(3) (3,3′-Diethyloxacarbocyanine, iodide) has been used for measuring membrane potentials in bacteria. The green fluorescent dye forms red fluorescent aggregates with increasing membrane potential. The spectral shifting phenomenon has been exploited to measure membrane potential ratiometrically.

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DiIC1(5) iodide mitochondria dye, for fluorescence microscopy

Supplier: Biotium

The carbocyanine dye DiIC1(5) has been used to measure mitochondrial membrane potential in apoptotic cells. Loss of membrane potential in apoptotic cells is reflected by a decrease of fluorescence signal in the infrared channel.

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MitoView™ 663 mitochondria dye, for fluorescence microscopy

MitoView™ 663 mitochondria dye, for fluorescence microscopy

Supplier: Biotium

MitoView™ dyes are fluorogenic mitochondrial stains for live cells. The dyes rapidly accumulate in mitochondria and can be imaged without washing. They are available with blue, green, far-red, and near-infrared fluorescence.

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Tris(bathophenanthrolinedisulphonate)ruthenium(II) sodium salt fluorescent dye, for histology

Tris(bathophenanthrolinedisulphonate)ruthenium(II) sodium salt fluorescent dye, for histology

Supplier: Biotium

RuBPS (Ruthenium(tris(bathophenanthrolinedisulfonate), tetrasodium salt) is a negatively-charged water-soluble luminescent ruthenium complex. The complex can be efficiently excited by either UV light or visible light in the wavelength range from 400 nm to 500 nm with bright red emission centered around 605 nm.

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Biotin-rhodamine 110 fluorescent dye, for histology

Biotin-rhodamine 110 fluorescent dye, for histology

Supplier: Biotium

Biotium developed biotin-rhodamine 110 as an alternative to biotin-4-fluorescein (90062) and fluorescein biotin (80019), both of which have been used for detection of biotin binding sites and the degree of biotinylation of proteins, and for the measurement of avidin and streptavidin in crude biological samples.

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RuBP-2S fluorescent dye, for histology

RuBP-2S fluorescent dye, for histology

Supplier: Biotium

RuBP-2S (Ruthenium(bis(bathophenanthroline))(bathophenanthrolinedisulfonate) is a luminescent ruthenium complex similar to RuBPS (80021). Two of the bathophenanthroline ligands are neutral while the third bathophenanthroline ligand is bis-sulfonated, resulting in an overall neutral charge of the ruthenium complex.

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