"Biotium"
DMAO 2 mM in DMSO used in nuclear staining
Supplier: Biotium
DMAO, 2 mM in DMSO is a green-fluorescent nucleic acid dye for staining live bacteria.
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7-Aminoactinomycin D (7-AAD)
Supplier: Biotium
7-Aminoactinomycin D (7-AAD)
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Actinomycin D ≥98% (by HPLC)
Supplier: Biotium
Actinomycin D is a nonfluorescent GC-selective intercalator.
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3,3'-Diethyloxadicarbocyanine iodide (DODC), high purity
Supplier: Biotium
DODC (DiOC2(5)) has been reported to bind to triplex DNA as well as dimeric hairpin quadruplex DNA. Binding of the dye to triplex DNA quenches the fluorescence of the dye and red-shifts the dye absorption peak.
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3,3'-Diethylthiadicarbocyanine iodide [DiSC₂(5)] ≥95% (by TLC) DNA/RNA binding dye
Supplier: Biotium
DiSC2(5) (3,3′-Diethylthiadicarbocyanine, iodide) binds to DNA-DNA or PNA-DNA duplexes with a large blue-shift of the absorption spectrum. Binding of the dye to PNA-DNA hybrid, in particular, blue-shifts the dye absorption spectrum by as much as 115 nm, offering a visual method to detect nucleic acid hybridization.
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Ethidium bromide 10 mg/ml in aqueous solution
Supplier: Biotium
Ethidium bromide 10 mg/ml in aqueous solution
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4',6'-Diamidino-2-phenylindole dihydrochloride (DAPI dihydrochloride) 10 mg/mL in water used in nuclear staining
Supplier: Biotium
DAPI (4′,6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions.
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bisBenzimide H 33258 trihydrochloride (Hoechst 33258) 10 mg/mL in water
Supplier: Biotium
Hoechst dyes are cell membrane-permeant, minor groove binding blue fluorescent DNA stains. Hoechst 33258 and Hoechst 33342 are spectrally similar. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. They are typically used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.
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bisBenzimide H 33258 trihydrochloride pentahydrate (Hoechst 33258)
Supplier: Biotium
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Biotium also offers Hoechst 33258, 10 mg/ml in dH2O (#40044). Hoechst 33258 and Hoechst 33342 are spectrally similar. Hoechst 33528 is slightly more water soluble than Hoechst 33342, but both dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. They are typically used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.
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bisbenzimide H-33342 trihydrochloride (Hoechst 33342) 10 mg/mL in water
Supplier: Biotium
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258, but either dye can be used to stain live or fixed cell in buffer or medium at 1 ug/mL, with no wash step required.
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bisBenzimide H-33342 trihydrochloride trihydrate (Hoechst 33342)
Supplier: Biotium
Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains.
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Ethidium Homodimer III (EthD-III) used in nuclear staining
Supplier: Biotium
A red fluorescent dead cell stain for bacteria and mammalian cells
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BAPTA-AM ≥98% (by TLC) ion chelator, for calcium determination
Supplier: Biotium
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.
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BAPTA tetracesium salt ≥95% (by TLC) ion chelator, for calcium determination
Supplier: Biotium
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations.
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BAPTA tetrapotassium salt ≥95% (by TLC) ion chelator, for calcium determination
Supplier: Biotium
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium. Key advantages of these calcium chelators include relative insensitivity toward intracellular pH change and fast release of calcium.
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BAPTA tetrasodium salt ≥95% (by TLC) ion chelator, for calcium determination
Supplier: Biotium
BAPTA and its derivatives are calcium chelators that are commonly used to form calcium buffers with well-defined calcium concentrations. By injecting the chelators into cells or by incubating cells with the AM ester form of the chelators, one can control the cytosolic calcium concentration, an important means to study the roles of calcium.