Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: HyClone products (Cytiva)

Supplements cell culture with concentrated amino acids. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

Supplier: Biowest

L-glutamine is an essential amino acid required by virtually all mammalian and insect cells grown in culture. It is a crucial component of many cell culture media and serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. However, in liquid media or stock solutions, L-glutamine can degrade relatively rapidly. L-glutamine is also more labile in cell culture solution than other amino acids.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, glucose, trace elements, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, glucose, and trace elements and manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified Amino Acids have been optimized for growth of embryonic cell cultures.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, salts, trace elements, and glucose. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: HyClone products (Cytiva)

Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: Biowest

Ham's F14 medium was developed from Ham's F12 medium, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham's F14 contains a double concentration of amino acids compared to the Ham's F12 medium. This product is also supplemented with calcium chloride and ascorbic acid.

Supplier: Biowest

The Leibovitz L-15 media were formulated to promote the cell growth in medium not balanced in CO₂. The formulations were developed with the sodium bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts, free base amino acids and galactose so they can be used under conditions of free gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses also have been successfully cultivated in this medium.

Supplier: HyClone products (Cytiva)

Minimum Essential Medium (MEM) is one of the earliest modifications of Basal Medium Eagle (BME) containing amino acid concentrations that more closely mimic those found in mammalian cells. MEM has been used with serum supplementation on a broad range of cell types including suspension and adherent mammalian cells, HeLa, BHK-21, HEK-293, HEp-2, HT-1080, MCF-7,fibroblasts and primary rat astrocytes.

Supplier: Biowest

In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium. Iscove's medium is a modification of Dulbecco's Modified Eagle's Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies have demonstrated that Iscove's medium will support murine B lymphocytes, haemopoietic tissue from bone marrow, B-cells stimulated with lipopolysaccharide, T-lymphocytes, and a variety of hybrid cells.

Supplier: HyClone products (Cytiva)

Ham's F-12 media was originally derived by modifying Ham's F-10 to include increased concentrations of choline, inositol, putrescine, and several amino acids. It was developed specifically for serum free culturing of Chinese Hamster Ovary (CHO) cells and has also been used with supplementation to grow chondrocytes, primary rat hepatocytes, rat prostate epithelial cells, carcinoma cells, rat skeletal myoblasts and rat, rabbit, and chicken embryos.

Supplier: Corning

The addition of MEM nonessential amino acids to medium can improve growth and viability of propagating cells in culture while reducing the biosynthetic burden on these cells.

Supplier: Corning

The addition of MEM nonessential amino acids to medium can improve growth and the viability of propagating cells in culture while reducing the biosynthetic burden on these cells.

Supplier: Corning

L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Supplier: MP Biomedicals

Most cells can synthesise non-essential amino acids (NEAA) through glucoaminolysis, glycolysis, or the TCA cycle. When these amino acids concentration levels are low, cells need to consume more glucose and glutamine in the media and produce excess by-products, which might affect cell growth. Adding NEAA into the cell culture medium could mitigate the potential side effects caused by self-production of these non-essential amino acids.

Supplier: MP Biomedicals

L-serine is used in the study of influence of pulsed applications of serine on polyglucose synthesis by Fusobacterium nucleatum cultures. Supplementation of minimal media with serine has been shown to restore growth in Escherichia coli that lack the PII and GlnK genes.

Supplier: US Biological

Drop-out mixes are used as a supplement to Drop-out base for making synthetic, complete media formulations in accordance with methods in yeast genetics. All lots are quality control tested using the appropriate yeast strains and transformants.

Supplier: Corning

L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use. This formulation is prepared in cell culture grade water.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C)
L-Glutamine, the uncharged and amidated analog of L-glutamic acid, is an important amino acid for the incorporation of NH4+ into biomolecules. It is biosynthesized from NH4.
L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture.
L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Supplier: Corning

L-glutamine is an essential amino acid and a key component of culture media, serving as a major energy source for propagating cells. It is very stable as a dry powder and as a frozen solution but degrades rapidly in liquid media or stock solutions, producing toxic compounds. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use. This formulation is prepared in cell culture grade water.

Supplier: HyClone products (Cytiva)

DMEM Low Glucose was the original modification of Basal Medium Eagle (BME) used to grow mouse embryonic cells. DMEM, Low Glucose is suitable for the culturing of many immortalised cell lines from humans, monkeys, hamsters, rats, mice, chicken and fish. DMEM contains four times more amino acids, vitamins and other supplementary components than Basal Medium Eagle (BME), the base media from which DMEM was derived. As DMEM contains no growth-promoting agents or proteins, supplementation with FBS is recommended for optimal performance.

Supplier: US Biological

RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C). Store Desiccated. Store under nitrogen.
L-Cysteine is a proteinogenic amino acid incorporated into proteins as directed by the genetic code. The thiol-side chain participates in a variety of oxidation/reduction reactions within the cell. The side chain participates in the formation of β bonds that modulate the secondary and ternary structure of proteins. Cysteine is essential and limiting for the formation of glutathione, an important antioxidant, within the cell.
Cysteine has been used in pulse-chase experiments to study protein expression from an inducible promoter. L-Cysteine is used to supplement cell culture media.
NMDA glutamatergic receptor agonist.

Supplier: STEMCELL Technologies

For hydrolysis of hyaluronic acid.