Human eNOS ELISA Kit

Human eNOS ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human eNOS in serum, plasma, tissue homogenates, cell lysates or other biological fluids.

Human eNOS ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human eNOS in serum, plasma, tissue homogenates, cell lysates or other biological fluids. An antibody specific for eNOS has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the eNOS present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-eNOS Antibody, which binds the captured eNOS present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of eNOS captured in each well. The concentration of eNOS can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Caution: This product is for Research Use Only. It is not for use in diagnostic or therapeutic procedures.