Fluorescamine, white powder

Fluorescamine is a non-fluorescent reagent that reacts readily with primary amino groups in amino acids and peptides to form highly fluorescent derivatives, whereas the reagent and its degradation products are non-fluorescent. No significant fluorescence with ammonia. Low background due to hydrolysis.
an plasma and urine and sulfonamides in honey by HPLC with fluorescence detection.

• Fluorescence: Emmax: 475 nm; Exc.: 390 nm in 0.5 M borate buffer, pH 8.5, after derivatization with L-leucine

Fluorescamine is a reagent for fluorescent assay of amino acids, peptides, proteins and other primary amines. It is also used for fluorometric assays of some proteolytic enzymes. It effectively blocks newly generated amino termini in protein sequence analyses by the Beckman automated sequencer. This product has been used in labeling casein so that it can be used as a substrate for measuring protease activity.

Fluorescamine is used in many sensitive detection methods, eg. characterization of poly-L-lysine (pLL)/DNA complexes post-modified with a multivalent hydrophilic polymer, spectrofluorimetric analysis of procaine, detection and quantitation of residual aminopenicillins by HPLC after fluorescamine derivation, determination of lisinopril in hum

Soluble in CH₂Cl₂, trichloromethane, chloroform, dimethysulphoxide; slightly soluble in acetone (50 mg/ml), ethanol and water.