3,3',5,5'-Tetramethylbenzidine free base

TMB is a widely used chromogenic substrate for horseradish peroxidase (HRP) in colorimetric assays. As a free base, it is typically dissolved in an organic solvent or buffer to prepare TMB substrate solutions for ELISA, Western blotting, and immunohistochemistry.

• Sensitive HRP Substrate: Enables high-sensitivity detection in enzyme-linked assays
• Colorimetric Detection: Produces a strong blue signal, with a yellow endpoint upon acid stop for dual-wavelength measurement
• Flexible Formulation: Free base form allows custom preparation for specific assay conditions
• Versatile Applications: Ideal for ELISA, Western blotting, and immunoassays in diagnostics and research
• Low Background: Provides high signal-to-noise ratio for accurate quantification