Sample clean up, Perfect-FOCUS™

Perfect-FOCUS™ is based on quantitative precipitation and concentration of protein solutions using UPPA™ (Universal Protein Precipitation Agent) and has been specifically developed for preparing lower conductivity protein samples for iso-electric focusing gels. Perfect-FOCUS™ is designed to clean and remove interfering agents from protein samples and concentrate protein solutions that give poor protein spot resolution during 2D electrophoresis.

Protein solutions as dilute as 1 ng/ml, if treated with Perfect-FOCUS™, can be quantitatively precipitated into a small volume. Protein precipitation is not affected by the presence of detergents or other common laboratory agents.

The collected protein sample has a conductivity of approximately 40 to 50 µS and is substantially free from non-protein agents, making the protein sample ideal for critical IEF and 2D gel electrophoresis studies, with improved spot resolution and greatly reduced spot streaking. Perfect-FOCUS™ treated protein samples are compatible with mass spectrometry analysis and show identical peptide spectra.